Mouse PGP9.5 (PGP9.5) Antibody Pair (STJAP00813)
General Information
| Short Description | This PGP9.5 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the. |
| Applications | ELISA |
| Host | Rabbit/Rabbit |
| Reactivity | Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Monoclonal |
| Clone ID | R25-8Q-9/R51-9Q-1 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | Determined by end user |
| Formulation | 0.01M PBS, pH 7.2 |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | Uchl1 |
| Gene ID | 22223 |
| Uniprot ID | UCHL1_MOUSE |
| Immunogen | Recombinant protein |
Additional Info
| Tissue Specificity | Expressed in brain, where it is found in neurons but not in oligodendrocytes or astrocytes. Found in the ganglion cell layer and the inner nuclear layer of the retina (at protein level). Expressed in brain and testis. In the brain, expression is at its lowest in replaceable neurons of hippocampus and olfactory bulb. Highly expressed in senescent pituitary. In skeletal muscle, primarily expressed in oxidative muscle fibers. |
| Post Translational Modifications | O-glycosylated. |
| Function | Deubiquitinase that plays a role in the regulation of several processes such as maintenance of synaptic function, cardiac function, inflammatory response or osteoclastogenesis. Abrogates the ubiquitination of multiple proteins including WWTR1/TAZ, EGFR, HIF1A and beta-site amyloid precursor protein cleaving enzyme 1/BACE1. In addition, recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin to maintain a stable pool of monoubiquitin that is a key requirement for the ubiquitin-proteasome and the autophagy-lysosome pathways. Regulates amyloid precursor protein/APP processing by promoting BACE1 degradation resulting in decreased amyloid beta production. Plays a role in the immune response by regulating the ability of MHC I molecules to reach cross-presentation compartments competent for generating Ag-MHC I complexes. Mediates the 'Lys-48'-linked deubiquitination of the transcriptional coactivator WWTR1/TAZ leading to its stabilization and inhibition of osteoclastogenesis. Deubiquitinates and stabilizes epidermal growth factor receptor EGFR to prevent its degradation and to activate its downstream mediators. Modulates oxidative activity in skeletal muscle by regulating key mitochondrial oxidative proteins. Enhances the activity of hypoxia-inducible factor 1-alpha/HIF1A by abrogateing its VHL E3 ligase-mediated ubiquitination and consequently inhibiting its degradation. |
| Protein Name | Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L1Uch-L1Neuron Cytoplasmic Protein 9.5Pgp 9.5Pgp9.5Ubiquitin Thioesterase L1 |
| Database Links | Reactome: R-MMU-5689603 |
| Cellular Localisation | CytoplasmEndoplasmic Reticulum MembraneLipid-AnchorLocalizes Near And/Or Within Mitochondria To Potentially Interact With Mitochondrial Proteins |
| Alternative Antibody Pair Names | Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L1 antibody pairUch-L1 antibody pairNeuron Cytoplasmic Protein 9.5 antibody pairPgp 9.5 antibody pairPgp9.5 antibody pairUbiquitin Thioesterase L1 antibody pairUchl1 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Mouse PGP9.5 (PGP9.5) Antibody Pair (STJAP00813)
This antibody pair detects mouse PGP9.5 (Ubiquitin C-terminal hydrolase L1, UniProt Q9R0P9) and is validated for reactivity in mouse samples only.
Both antibodies are rabbit monoclonal IgGs supplied unconjugated at 1 mg/mL in 0.01M PBS, pH 7.2, and are affinity purified. The clone IDs are R25-8Q-9 and R51-9Q-1 respectively.
This pair is validated for use in ELISA. No other assay formats are listed in the product specifications.
Working concentrations are determined by the end user and are not defined in the supplied product specifications. Titration experiments are recommended to establish optimal conditions for your specific assay setup.
Both antibodies should be stored at 4°C for short-term use. For long-term storage, aliquot and store at -20°C. Repeated freeze/thaw cycles should be avoided to maintain antibody integrity.
Both antibodies in this pair were raised against a recombinant mouse PGP9.5 protein. No specific immunogen region is defined in the product specifications.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.