Mouse PDHa (Pyruvate Dehydrogenase Alpha) Sandwich ELISA Kit Ready-To-Use Kit (STJE0017754)
SPECIFICATIONS
General Information
| Short Description | This PDHa Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse serum, plasma, tissue homogenates, cell lysates or other biological fluids.. |
| Applications | ELISA |
| Reactivity | Mouse |
| Sensitivity | 0.042ng/mL |
| Detection Limit | 0.156-10ng/mL |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Storage Instruction | The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time | 3 hrs |
Target Information
| Gene Symbol | Pdha1 |
| Gene ID | 18597 |
| Uniprot ID | ODPA_MOUSE |
| Immunogen Region | Ready-To-Use |
| Sample Type | serum, plasma, tissue homogenates, cell lysates or other biological fluids. |
Additional Info
| Tissue Specificity | In all tissues, but in very low amount in testis. |
| Post Translational Modifications | Phosphorylation at Ser-232, Ser-293 and Ser-300 by PDK family kinases inactivates the enzyme.for this phosphorylation at a single site is sufficient. Phosphorylation at Ser-293 interferes with access to active site, and thereby inactivates the enzyme. Dephosphorylation at all three sites, i.e. at Ser-232, Ser-293 and Ser-300, is required for reactivation. Acetylation alters the phosphorylation pattern. Deacetylated by SIRT3. |
| Function | The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle. |
| Protein Name | Pyruvate Dehydrogenase E1 Component Subunit Alpha - Somatic Form - MitochondrialPdhe1-A Type I |
| Database Links | Reactome: R-MMU-204174Reactome: -MMU-5362517Reactome: -MMU-9837999Reactome: -MMU-9861559 |
| Cellular Localisation | Mitochondrion Matrix |
| Alternative ELISA Names | Pyruvate Dehydrogenase E1 Component Subunit Alpha - Somatic Form - Mitochondrial ELISA kitPdhe1-A Type I ELISA kitPdha1 ELISA kitPdha-1 ELISA kit |
| output |
Information sourced from Uniprot.org