Mouse PCSK9 (Proprotein Convertase Subtilisin/Kexin Type 9) Antibody Pair (STJAP00523)
General Information
| Short Description | This PCSK9 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Rabbit/Rabbit |
| Reactivity | Mouse |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Antigen Affinity.Detection Purification: Antigen Affinity. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300; 50% glycerol; pH 7.5.Detection Buffer: PBS with 0.04% Proclin 300; 1% protective protein; 50% glycerol; pH 7.5. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
| Determination Method | Colormetric |
Target Information
| Gene Symbol | Pcsk9 |
| Gene ID | 100102 |
| Uniprot ID | PCSK9_MOUSE |
| Immunogen | Capture immunogen: Recombinant Mouse PCSK9 protien.Detection Immunogen: Recombinant Mouse PCSK9 protien. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Post Translational Modifications | Cleavage by furin and PCSK5 generates a truncated inactive protein that is unable to induce LDLR degradation. Undergoes autocatalytic cleavage in the endoplasmic reticulum to release the propeptide from the N-terminus and the cleavage of the propeptide is strictly required for its maturation and activation. The cleaved propeptide however remains associated with the catalytic domain through non-covalent interactions, preventing potential substrates from accessing its active site. As a result, it is secreted from cells as a propeptide-containing, enzymatically inactive protein. Phosphorylation protects the propeptide against proteolysis. |
| Function | Crucial player in the regulation of plasma cholesterol homeostasis. Binds to low-density lipid receptor family members: low density lipoprotein receptor (LDLR), very low density lipoprotein receptor (VLDLR), apolipoprotein E receptor (LRP1/APOER) and apolipoprotein receptor 2 (LRP8/APOER2), and promotes their degradation in intracellular acidic compartments. Acts via a non-proteolytic mechanism to enhance the degradation of the hepatic LDLR through a clathrin LDLRAP1/ARH-mediated pathway. May prevent the recycling of LDLR from endosomes to the cell surface or direct it to lysosomes for degradation. Can induce ubiquitination of LDLR leading to its subsequent degradation. Inhibits intracellular degradation of APOB via the autophagosome/lysosome pathway in a LDLR-independent manner. Involved in the disposal of non-acetylated intermediates of BACE1 in the early secretory pathway. Inhibits epithelial Na(+) channel (ENaC)-mediated Na(+) absorption by reducing ENaC surface expression primarily by increasing its proteasomal degradation. Regulates neuronal apoptosis via modulation of LRP8/APOER2 levels and related anti-apoptotic signaling pathways. |
| Protein Name | Proprotein Convertase Subtilisin/Kexin Type 9Neural Apoptosis-Regulated Convertase 1Narc-1Proprotein Convertase 9Pc9Subtilisin/Kexin-Like Protease Pc9 |
| Database Links | Reactome: R-MMU-381426Reactome: -MMU-8866427Reactome: -MMU-8957275Reactome: -MMU-8964038 |
| Cellular Localisation | CytoplasmSecretedEndosomeLysosomeCell SurfaceEndoplasmic ReticulumGolgi ApparatusAutocatalytic Cleavage Is Required To Transport It From The Endoplasmic Reticulum To The Golgi Apparatus And For The Secretion Of The Mature ProteinLocalizes To The Endoplasmic Reticulum In The Absence Of Ldlr And Co-Localizes To The Cell Surface And To The Endosomes/Lysosomes In The Presence Of LdlrThe Sorting To The Cell Surface And Endosomes Is Required In Order To Fully Promote Ldlr Degradation |
| Alternative Antibody Pair Names | Proprotein Convertase Subtilisin/Kexin Type 9 antibody pairNeural Apoptosis-Regulated Convertase 1 antibody pairNarc-1 antibody pairProprotein Convertase 9 antibody pairPc9 antibody pairSubtilisin/Kexin-Like Protease Pc9 antibody pairPcsk9 antibody pairNarc1 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Mouse PCSK9 (Proprotein Convertase Subtilisin/Kexin Type 9) Antibody Pair (STJAP00523)
This antibody pair detects mouse PCSK9 (Proprotein Convertase Subtilisin/Kexin Type 9), corresponding to UniProt entry Q80W65. Reactivity is confirmed for mouse samples only.
The capture antibody is supplied unconjugated and the detection antibody is biotinylated. Both are rabbit-raised, antigen affinity-purified antibodies supplied at 0.5 mg/mL.
This antibody pair has been validated for use in ELISA. It is suitable for use with serum, plasma, and other biological fluids.
The capture antibody is formulated in PBS with 0.04% Proclin 300 and 50% glycerol at pH 7.5. The detection antibody is formulated in PBS with 0.04% Proclin 300, 1% protective protein, and 50% glycerol at pH 7.5.
Both components should be stored at -20°C for long-term storage. Freeze-thaw cycles should be avoided to maintain product integrity.
Both the capture and detection antibodies were raised against recombinant mouse PCSK9 protein. Both antibodies were subsequently purified by antigen affinity methods.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.