Mouse OPN (Osteopontin) Sandwich ELISA Kit (STJE0007148)
SPECIFICATIONS
General Information
| Short Description | This OPN Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.. |
| Applications | ELISA |
| Reactivity | Mouse |
| Sensitivity | 16pg/mL |
| Detection Limit | 39-2500pg/mL |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Storage Instruction | Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time | 4.5 hrs |
Target Information
| Gene Symbol | Spp1 |
| Gene ID | 20750 |
| Uniprot ID | OSTP_MOUSE |
| Sample Type | serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Additional Info
| Tissue Specificity | |
| Post Translational Modifications | Extensively phosphorylated by FAM20C in the extracellular medium at multiple sites within the S-x-E/pS motif. The phosphorylated form inhibits hydroxyapatite crystallization. Dephosphorylation via a mechanism involving ALPL/TNAP promotes hydroxyapatite crystallization. O-glycosylated. Forms covalent cross-links mediated by transglutaminase TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers, increasing its collagen binding properties. |
| Function | Major non-collagenous bone protein that binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity. |
| Protein Name | Osteopontin2arBone Sialoprotein 1Calcium Oxalate Crystal Growth Inhibitor ProteinEarly T-Lymphocyte Activation 1 ProteinMinopontinSecreted Phosphoprotein 1Spp-1 |
| Database Links | Reactome: R-MMU-1474228Reactome: -MMU-186797Reactome: -MMU-216083Reactome: -MMU-381426Reactome: -MMU-8957275 |
| Cellular Localisation | Secreted |
| Alternative ELISA Names | Osteopontin ELISA kit2ar ELISA kitBone Sialoprotein 1 ELISA kitCalcium Oxalate Crystal Growth Inhibitor Protein ELISA kitEarly T-Lymphocyte Activation 1 Protein ELISA kitMinopontin ELISA kitSecreted Phosphoprotein 1 ELISA kitSpp-1 ELISA kitSpp1 ELISA kitEta-1 ELISA kitOp ELISA kitSpp-1 ELISA kit |
| output |
Information sourced from Uniprot.org