Mouse OCT4 (Octamer Binding Transcription Factor 4) Sandwich ELISA Kit Ready-To-Use Kit (STJE0017558)
SPECIFICATIONS
General Information
| Short Description | This OCT4 Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse tissue homogenates or other biological fluids.. |
| Applications | ELISA |
| Reactivity | Mouse |
| Sensitivity | 0.057ng/mL |
| Detection Limit | 0.156-10ng/mL |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Storage Instruction | The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time | 3 hrs |
Target Information
| Gene Symbol | Pou5f1 |
| Gene ID | 18999 |
| Uniprot ID | PO5F1_MOUSE |
| Immunogen Region | Ready-To-Use |
| Sample Type | tissue homogenates or other biological fluids. |
Additional Info
| Tissue Specificity | Expressed the totipotent and pluripotent stem cells of the pregastrulation embryo. Also expressed in primordial germ cells and in the female germ line. Absent from adult tissues. |
| Post Translational Modifications | Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression. Ubiquitinated.undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation. ERK1/2-mediated phosphorylation at Ser-106 promotes nuclear exclusion and proteasomal degradation. Phosphorylation at Thr-228 and Ser-229 decrease DNA-binding and alters ability to activate transcription. JNK1/2-mediated phosphorylation at Ser-347 promotes proteasomal degradation. |
| Function | Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 or SOX15 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency. |
| Protein Name | Pou Domain - Class 5 - Transcription Factor 1Nf-A3Octamer-Binding Protein 3Oct-3Octamer-Binding Protein 4Oct-4Octamer-Binding Transcription Factor 3Otf-3 |
| Database Links | |
| Cellular Localisation | CytoplasmNucleusExpressed In A Diffuse And Slightly Punctuate PatternColocalizes With Mapk8 And Mapk9 In The Nucleus |
| Alternative ELISA Names | Pou Domain - Class 5 - Transcription Factor 1 ELISA kitNf-A3 ELISA kitOctamer-Binding Protein 3 ELISA kitOct-3 ELISA kitOctamer-Binding Protein 4 ELISA kitOct-4 ELISA kitOctamer-Binding Transcription Factor 3 ELISA kitOtf-3 ELISA kitPou5f1 ELISA kitOct-3 ELISA kitOct-4 ELISA kitOtf-3 ELISA kitOtf3 ELISA kit |
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Information sourced from Uniprot.org