Mouse NGF (Beta-Nerve Growth Factor) Antibody Pair (STJAP00288)
General Information
| Short Description | This NGF antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Rabbit/Rabbit |
| Reactivity | Mouse |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Polyclonal |
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Protein A or G.Detection Purification: Protein A & Antigen Affinity. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4.Detection Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
Target Information
| Gene Symbol | Ngf |
| Gene ID | 18049 |
| Uniprot ID | NGF_MOUSE |
| Immunogen | Capture immunogen: Recombinant Mouse NGF protein.Detection Immunogen: Recombinant Mouse NGF protein. |
Additional Info
| Function | Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades to regulate neuronal proliferation, differentiation and survival. The immature NGF precursor (proNGF) functions as a ligand for the heterodimeric receptor formed by SORCS2 and NGFR, and activates cellular signaling cascades that lead to inactivation of RAC1 and/or RAC2, reorganization of the actin cytoskeleton and neuronal growth cone collapse. In contrast to mature NGF, the precursor form (proNGF) promotes neuronal apoptosis (in vitro). Inhibits metalloproteinase-dependent proteolysis of platelet glycoprotein VI. Binds lysophosphatidylinositol and lysophosphatidylserine between the two chains of the homodimer. The lipid-bound form promotes histamine relase from mast cells, contrary to the lipid-free form. |
| Protein Name | Beta-Nerve Growth FactorBeta-Ngf |
| Database Links | Reactome: R-MMU-167060Reactome: -MMU-170968Reactome: -MMU-170984Reactome: -MMU-177504Reactome: -MMU-187042Reactome: -MMU-198203Reactome: -MMU-205017Reactome: -MMU-205025Reactome: -MMU-205043Reactome: -MMU-209543Reactome: -MMU-209560Reactome: -MMU-209563 |
| Cellular Localisation | SecretedEndosome LumenProngf Is Endocytosed After Binding To The Cell Surface Receptor Formed By Sort1 And Ngfr |
| Alternative Antibody Pair Names | Beta-Nerve Growth Factor antibody pairBeta-Ngf antibody pairNgf antibody pairNgfb antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Mouse NGF (Beta-Nerve Growth Factor) Antibody Pair (STJAP00288)
This antibody pair is designed to detect mouse Beta-Nerve Growth Factor (NGF), corresponding to UniProt entry P01139. Reactivity has been confirmed for mouse only.
The capture antibody is an unconjugated rabbit monoclonal antibody, and the detection antibody is a biotin-conjugated rabbit polyclonal antibody. Both are supplied at 0.5 mg/mL in PBS with 0.04% Proclin 300 and 50% glycerol at pH 7.4.
This antibody pair has been validated for use in ELISA. No other assay formats are listed in the product specifications.
The capture antibody was purified by Protein A or G, and the detection antibody was purified by Protein A combined with antigen affinity purification. Both antibodies were raised against recombinant mouse NGF protein.
Both components should be stored at -20°C for long-term storage. Freeze-thaw cycles should be avoided to maintain reagent integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.