Mouse NGAL (Neutrophil Gelatinase-Associated Lipocalin) Antibody Pair (STJAP00080)
General Information
| Short Description | This NGAL antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Goat/Goat |
| Reactivity | Mouse |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Clonality | Polyclonal/Polyclonal |
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Antigen Affinity.Detection Purification: Antigen Affinity. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4.Detection Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
Target Information
| Gene Symbol | Lcn2 |
| Gene ID | 16819 |
| Uniprot ID | NGAL_MOUSE |
| Immunogen | Capture immunogen: Recombinant Mouse NGAL protein.Detection Immunogen: Recombinant Mouse NGAL protein. |
Additional Info
| Post Translational Modifications | N-glycosylated. |
| Function | Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,3-dihydroxybenzoic acid (2,3-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity.limits bacterial proliferation by sequestering iron bound to microbial siderophores, such as enterobactin. Can also bind siderophores from M.tuberculosis. |
| Protein Name | Neutrophil Gelatinase-Associated LipocalinNgalLipocalin-2Oncogene 24p324p3Sv-40-Induced 24p3 ProteinSiderocalin Lcn2P25 |
| Database Links | Reactome: R-MMU-6798695Reactome: -MMU-6799990Reactome: -MMU-917937 |
| Cellular Localisation | SecretedCytoplasmic Granule LumenCytoplasmic Vesicle LumenUpon Binding To The Slc22a17 (24p3r) ReceptorIt Is InternalizedReleases The Bound Iron In The Acidic Lumen Of Cytoplasmic Vesicles |
| Alternative Antibody Pair Names | Neutrophil Gelatinase-Associated Lipocalin antibody pairNgal antibody pairLipocalin-2 antibody pairOncogene 24p3 antibody pair24p3 antibody pairSv-40-Induced 24p3 Protein antibody pairSiderocalin Lcn2 antibody pairP25 antibody pairLcn2 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Mouse NGAL (Neutrophil Gelatinase-Associated Lipocalin) Antibody Pair (STJAP00080)
This antibody pair detects Mouse NGAL (Neutrophil Gelatinase-Associated Lipocalin), corresponding to UniProt P11672, and is validated for use with mouse samples only.
Both antibodies are polyclonal, raised in goat, and supplied at 0.5 mg/mL. The capture antibody is unconjugated, while the detection antibody is biotinylated. Both are formulated in PBS with 0.04% Proclin 300 and 50% glycerol at pH 7.4.
This antibody pair is validated for ELISA only. No other assay formats are listed in the product specifications.
Both the capture and detection antibodies were raised against recombinant Mouse NGAL protein. Both were subsequently purified by antigen affinity chromatography.
Both components should be stored at -20°C for long-term storage. Freeze-thaw cycles should be avoided to preserve antibody integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.