Mouse NEFL (Neurofilament, Light Polypeptide) Sandwich ELISA Kit (STJE0019815)

Name: Mouse NEFL (Neurofilament, Light Polypeptide) Sandwich ELISA Kit (STJE0019815)
SKU: STJE0019815
Availability: InStock

⇓ Datasheet ⇓ SDS

SPECIFICATIONS

STJE0019815

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Short Description	This NEFL Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse serum, plasma and other biological fluids.
Applications	ELISA
Reactivity	Mouse
Sensitivity	0.1ng/mL
Detection Limit	0.16~10ng/mL
Note	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Storage Instruction	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time	3.5h
Detection	Colormetric

Gene Symbol	Nefl
Gene ID	18039
Uniprot ID	NFL_MOUSE
Specificity	This kit recognizes Mouse NEFL in samples. No significant crossreactivity or interference between Mouse NEFL and analogues was observed.
Sample Type	serum, plasma and other biological fluids

Tissue Specificity	Expressed in the sciatic nerve (at protein level).
Post Translational Modifications	O-glycosylated. Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization. Ubiquitinated in the presence of TRIM2 and UBE2D1.
Function	Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks.
Protein Name	Neurofilament Light Polypeptide Nf-L 68 Kda Neurofilament Protein Neurofilament Triplet L Protein
Database Links	Reactome: R-MMU-438066 Reactome: -MMU-5673001
Cellular Localisation	Cell Projection Axon Cytoplasm Cytoskeleton
Alternative ELISA Names	Neurofilament Light Polypeptide ELISA kit Nf-L ELISA kit 68 Kda Neurofilament Protein ELISA kit Neurofilament Triplet L Protein ELISA kit Nefl ELISA kit Nf68 ELISA kit Nfl ELISA kit
Specificity	This kit recognizes Mouse NEFL in samples. No significant cross-reactivity or interference between Mouse NEFL and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (ng/mL)	0.46	1.57	3.52	0.46	1.60	3.60
Standard deviation	0.02	0.08	0.18	0.03	0.08	0.12
CV (%)	4.35	5.10	5.11	6.52	5.00	3.33