Mouse N Cadherin (N Cadherin) Antibody Pair (STJAP00797)
General Information
| Short Description | This N Cadherin antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the. |
| Applications | ELISA |
| Host | Rabbit/Rabbit |
| Reactivity | Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Monoclonal |
| Clone ID | R53-6Y-2/R49-7X-7 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | Determined by end user |
| Formulation | 0.01M PBS, pH 7.2 |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | Cdh2 |
| Gene ID | 12558 |
| Uniprot ID | CADH2_MOUSE |
| Immunogen | Recombinant protein |
Additional Info
| Tissue Specificity | Expressed in cardiac muscle (at protein level). |
| Post Translational Modifications | Cleaved by MMP24. Ectodomain cleavage leads to the generation of a soluble 90 kDa N-terminal soluble fragment and a 45 kDa membrane-bound C-terminal fragment 1 (CTF1), which is further cleaved by gamma-secretase into a 35 kDa. Cleavage in neural stem cells by MMP24 affects CDH2-mediated anchorage of neural stem cells to ependymocytes in the adult subependymal zone, leading to modulate neural stem cell quiescence. May be phosphorylated by OBSCN. O-glycosylated on Ser and Thr residues. |
| Function | Calcium-dependent cell adhesion protein.preferentially mediates homotypic cell-cell adhesion by dimerization with a CDH2 chain from another cell. Cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. Plays a role in cell-to-cell junction formation between pancreatic beta cells and neural crest stem (NCS) cells, promoting the formation of processes by NCS cells. Required for proper neurite branching. Required for pre- and postsynaptic organization. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density. |
| Protein Name | Cadherin-2Neural CadherinN-CadherinCd Antigen Cd325 |
| Database Links | Reactome: R-MMU-381426Reactome: -MMU-418990Reactome: -MMU-525793Reactome: -MMU-8957275 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinSarcolemmaCell JunctionAdherens JunctionDesmosomeCell SurfaceColocalizes With Tmem65 At The Intercalated Disk In CardiomyocytesColocalizes With Obscn At The Intercalated Disk And Sarcolemma In Cardiomyocytes |
| Alternative Antibody Pair Names | Cadherin-2 antibody pairNeural Cadherin antibody pairN-Cadherin antibody pairCd Antigen Cd325 antibody pairCdh2 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Mouse N Cadherin (N Cadherin) Antibody Pair (STJAP00797)
This antibody pair is designed to detect mouse N Cadherin (UniProt P15116) and has confirmed reactivity for mouse samples. It is intended for use in sandwich ELISA-based research applications.
Both antibodies are supplied as unconjugated, affinity-purified rabbit monoclonal IgGs (clones R53-6Y-2 and R49-7X-7) at 1 mg/mL, formulated in 0.01M PBS at pH 7.2. Neither antibody carries an enzyme or fluorescent conjugate, so the user is responsible for labeling the detection antibody or using a secondary detection system.
This pair is validated for ELISA. No other assay formats are listed in the product specifications.
Optimal working concentrations are determined by the end user and are not defined in the supplied product specifications. Titration experiments are recommended to establish the appropriate dilutions for each antibody within the user's specific experimental system.
Both antibodies should be stored at 4°C for short-term use. For long-term storage, aliquots should be kept at -20°C. Repeated freeze/thaw cycles should be avoided to preserve antibody integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.