Mouse Mydgf (Myeloid-derived growth factor) Sandwich ELISA Kit (STJE0004326)

Name: Mouse Mydgf (Myeloid-derived growth factor) Sandwich ELISA Kit (STJE0004326)
SKU: STJE0004326
Availability: InStock

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SPECIFICATIONS

STJE0004326

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Short Description	This Mydgf Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse serum, plasma and other biological fluids.
Applications	ELISA
Reactivity	Mouse
Sensitivity	9.38pg/mL
Detection Limit	15.63~1000pg/mL
Note	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Storage Instruction	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time	3.5h
Detection	Colormetric

Gene Symbol	Mydgf
Gene ID	28106
Uniprot ID	MYDGF_MOUSE
Specificity	This kit recognizes Mouse IL-25 in samples. No significant cross-reactivity or interference between Mouse IL-25 and analogues was observed.
Sample Type	Serum, plasma and other biological fluids

Function	Bone marrow-derived monocyte and paracrine-acting protein that promotes cardiac myocyte survival and adaptive angiogenesis for cardiac protection and/or repair after myocardial infarction (MI). Stimulates endothelial cell proliferation through a MAPK1/3-, STAT3- and CCND1-mediated signaling pathway. Inhibits cardiac myocyte apoptosis in a PI3K/AKT-dependent signaling pathway.
Protein Name	Myeloid-Derived Growth Factor Mydgf
Cellular Localisation	Secreted Endoplasmic Reticulum-Golgi Intermediate Compartment Endoplasmic Reticulum Golgi Apparatus The C-Terminal Rtel Motif May Provide Retention In The Endoplasmic Reticulum
Alternative ELISA Names	Myeloid-Derived Growth Factor ELISA kit Mydgf ELISA kit Mydgf ELISA kit D17Wsu104e ELISA kit
Specificity	This kit recognizes Mouse IL-25 in samples. No significant cross-reactivity or interference between Mouse IL-25 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	50.94	140.25	422.86	55.24	134.27	436.28
Standard deviation	3.49	6.68	22.79	3.65	5.63	21.16
CV (%)	6.85	4.76	5.39	6.61	4.19	4.85