Mouse Ccl3 (C-C motif chemokine 3) Sandwich ELISA Kit (STJE0004207)

SPECIFICATIONS
STJE0004207
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General Information

Short DescriptionThis Ccl3 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse serum, plasma and other biological fluids.
ApplicationsELISA
ReactivityMouse
Sensitivity9.38pg/mL
Detection Limit15.63~1000pg/mL
NoteFOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Product Properties

Storage InstructionIf unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time3.5h
DetectionColormetric

Target Information

Gene SymbolCcl3
Gene ID20302
Uniprot IDCCL3_MOUSE
SpecificityThis kit recognizes Mouse MIP-1 Alpha in samples. No significant cross-reactivity or interference between Mouse MIP-1 Alpha and analogues was observed.
Sample TypeSerum, plasma and other biological fluids

Additional Info

Function Monokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV).
Protein Name C-C Motif Chemokine 3
Heparin-Binding Chemotaxis Protein
L2g25b
Macrophage Inflammatory Protein 1-Alpha
Mip-1-Alpha
Sis-Alpha
Small-Inducible Cytokine A3
Ty-5
Database Links Reactome: R-MMU-380108
Cellular Localisation Secreted
Alternative ELISA Names C-C Motif Chemokine 3 ELISA kit
Heparin-Binding Chemotaxis Protein ELISA kit
L2g25b ELISA kit
Macrophage Inflammatory Protein 1-Alpha ELISA kit
Mip-1-Alpha ELISA kit
Sis-Alpha ELISA kit
Small-Inducible Cytokine A3 ELISA kit
Ty-5 ELISA kit
Ccl3 ELISA kit
Mip1a ELISA kit
Scya3 ELISA kit
Specificity This kit recognizes Mouse MIP-1α in samples. No significant cross-reactivity or interference between Mouse MIP-1α and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 86-99 92
EDTA plasma (n=8) 90-103 95
Cell culture media (n=8) 89-105 96

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 46.90 154.80 386.60 42.70 160.00 368.20
Standard deviation 2.40 8.00 13.90 2.90 9.40 19.90
CV (%) 5.12 5.17 3.60 6.79 5.88 5.40

Citations

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