| Applications: | ELISA |
| Reactivity: | Mouse |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 9.38pg/mL |
| Detection Limit: | 15.63~1000pg/mL |
| Short Description : | This Ccl3 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse serum, plasma and other biological fluids. |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Colormetric |
| Gene Symbol: | Ccl3 |
| Gene ID: | 20302 |
| Uniprot ID: | CCL3_MOUSE |
| Specificity: | This kit recognizes Mouse MIP-1 Alpha in samples. No significant cross-reactivity or interference between Mouse MIP-1 Alpha and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
| Function | Monokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). |
| Protein Name | C-C Motif Chemokine 3 Heparin-Binding Chemotaxis Protein L2g25b Macrophage Inflammatory Protein 1-Alpha Mip-1-Alpha Sis-Alpha Small-Inducible Cytokine A3 Ty-5 |
| Database Links | Reactome: R-MMU-380108 |
| Cellular Localisation | Secreted |
| Alternative ELISA Names | C-C Motif Chemokine 3 ELISA kit Heparin-Binding Chemotaxis Protein ELISA kit L2g25b ELISA kit Macrophage Inflammatory Protein 1-Alpha ELISA kit Mip-1-Alpha ELISA kit Sis-Alpha ELISA kit Small-Inducible Cytokine A3 ELISA kit Ty-5 ELISA kit Ccl3 ELISA kit Mip1a ELISA kit Scya3 ELISA kit |
| Specificity | This kit recognizes Mouse MIP-1α in samples. No significant cross-reactivity or interference between Mouse MIP-1α and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 86-99 | 92 |
| EDTA plasma (n=8) | 90-103 | 95 |
| Cell culture media (n=8) | 89-105 | 96 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 46.90 | 154.80 | 386.60 | 42.70 | 160.00 | 368.20 |
| Standard deviation | 2.40 | 8.00 | 13.90 | 2.90 | 9.40 | 19.90 |
| CV (%) | 5.12 | 5.17 | 3.60 | 6.79 | 5.88 | 5.40 |