Mouse MFGE8 (Lactadherin) Antibody Pair (STJAP00572)
General Information
| Short Description | This MFGE8 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Goat/Goat |
| Reactivity | Mouse |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Antigen Affinity.Detection Purification: Antigen Affinity. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300; 50% glycerol; pH 7.5.Detection Buffer: PBS with 0.04% Proclin 300; 1% protective protein; 50% glycerol; pH 7.5. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
| Determination Method | Colormetric |
Target Information
| Gene Symbol | Mfge8 |
| Gene ID | 17304 |
| Uniprot ID | MFGM_MOUSE |
| Immunogen | Capture immunogen: Recombinant Mouse MFGE8 protien.Detection Immunogen: Recombinant Mouse MFGE8 protien. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Post Translational Modifications | N-glycosylated. Isoform 1 also exists in both an O-glycosylated and a non-O-glycosylated form. |
| Function | Contributes to phagocytic removal of apoptotic cells in many tissues. Specific ligand for the alpha-v/beta-3 and alpha-v/beta-5 receptors. Also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner. Zona pellucida-binding protein which may play a role in gamete interaction. Plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. Promotes VEGF-dependent neovascularization. |
| Protein Name | LactadherinMfgmMilk Fat Globule-Egf Factor 8Mfg-E8Sed1Sperm Surface Protein Sp47Mp47 |
| Database Links | Reactome: R-MMU-381426Reactome: -MMU-8957275 |
| Cellular Localisation | MembranePeripheral Membrane ProteinSecretedCytoplasmic VesicleSecretory VesicleAcrosome MembraneLocated In The Acrosomal Region Of Zona-Pellucida Bound Sperm |
| Alternative Antibody Pair Names | Lactadherin antibody pairMfgm antibody pairMilk Fat Globule-Egf Factor 8 antibody pairMfg-E8 antibody pairSed1 antibody pairSperm Surface Protein Sp47 antibody pairMp47 antibody pairMfge8 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Mouse MFGE8 (Lactadherin) Antibody Pair (STJAP00572)
This antibody pair detects mouse MFGE8, also known as Lactadherin, corresponding to UniProt entry P21956. Reactivity has been established for mouse samples only.
The capture antibody is supplied unconjugated at 0.5 mg/mL in PBS with 0.04% Proclin 300 and 50% glycerol at pH 7.5, while the detection antibody is biotinylated and supplied at 0.5 mg/mL in PBS with 0.04% Proclin 300, 1% protective protein, and 50% glycerol at pH 7.5. Both are goat-derived and purified by antigen affinity.
This antibody pair is validated for use in ELISA. It is intended for research use only and is not validated for diagnostic or therapeutic applications.
Validated sample types include serum, plasma, and other biological fluids from mouse origin.
Both the capture and detection antibodies were raised against recombinant mouse MFGE8 protein. Both were purified by antigen affinity chromatography.
Both components should be stored at -20°C for long-term storage. Freeze-thaw cycles should be avoided to maintain antibody integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.