Mouse LIF protein (Recombinant) (STJP000229)
SPECIFICATIONS
HostCHO Cells
ConjugationUnconjugated
ImmunogenOptimized DNA sequence encoding Mouse LIF mature chain was expressed in CHO Cells. NA
General Information
| Short Description | Recombinant-Mouse LIF-protein was developed from cho cells. For use in research applications. |
| Host | CHO Cells |
| Note | STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Dilution Range | Spin the vial and reconstite in distilled water to a concentration not less than 0.1 mg/mL. This can then be diluted into other buffers. |
| Formulation | Lyophilised from 0.2 Mu m filtered PBS pH.5. |
| Storage Instruction | Can be stored in working aliquots at°C-°C C for one month, or at-20°C C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. NA |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg (1EU/µg). NA |
| Immunoreactivity | The ED (50) was determined by the ability to inhibit differentiation of M1 myeloid leukemic cells was found to be in the range of.5 ng/ml. NA |
Target Information
| Gene Symbol | Lif |
| Gene ID | 16878 |
| Uniprot ID | LIF_MOUSE |
| Immunogen | Optimized DNA sequence encoding Mouse LIF mature chain was expressed in CHO Cells. NA |
| Immunogen Region | Mature chain |
| Immunogen Sequence | MKVLAAGIVP LLLLVLHWKH GAGSPLPITP VNATCAIRHP CHGNLMNQIK NQLAQLNGSA NALFISYYTA QGEPFPNNVE KLCAPNMTDF PSFHGNGTEK TKLVELYRMV AYLSASLTNI TRDQKVLNPT AVSLQVKLNA TIDVMRGLLS NVLCRLCNKY RVGHVDVPPV PDHSDKEAFQ RKKLGCQLLG TYKQVISVVV QAF NA |
Additional Info
| Function | LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes. |
| Protein Name | Leukemia Inhibitory FactorLifDifferentiation-Stimulating FactorD Factor |
| Database Links | Reactome: R-MMU-6788467 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | Leukemia Inhibitory Factor proteinLif proteinDifferentiation-Stimulating Factor proteinD Factor proteinLif protein |
Information sourced from Uniprot.org