Mouse Leptin/LEP protein (Recombinant) (C-His) (STJP003060)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Mouse Leptin/LEP Protein is produced by E. coli expression system. The target protein is expressed with sequence (Val22-Cys167) of mouse Leptin (Accession #NP_032519.1) fused with no additional amino acid.
General Information
| Short Description | Recombinant-Mouse Leptin/LEP-C-His protein was developed in hek293 cells using the region Val22-Cys167. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of 20mM PB, 300mM NaCl, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | Measured by its binding ability in a functional ELISA. Immobilized mouse Leptin/LEP at 0.5 Mu g/mL (100 Mu L/well) can bind human LEP-R/CD295 with a linear range of 0.34-70.6 ng/mL. |
| Determination Method | <1 EU/Mu g |
Target Information
| Gene Symbol | Lep |
| Gene ID | 16846 |
| Uniprot ID | LEP_MOUSE |
| Immunogen | Recombinant Mouse Leptin/LEP Protein is produced by E. coli expression system. The target protein is expressed with sequence (Val22-Cys167) of mouse Leptin (Accession #NP_032519.1) fused with no additional amino acid. |
| Immunogen Region | Val22-Cys167 |
| Immunogen Sequence | SLGCDLPQTHSLGNRRALIL LAQMRRISPFSCLKDRHDFE FPQEEFDDKQFQKAQAISVL HEMIQQTFNLFSTKDSSAAL DETLLDEFYIELDQQLNDLE SCVMQEVGVIESPLMYEDSI LAVRKYFQRITLYLTEKKYS SCAWEVVRAEIMRSFSLSIN LQKRLKSKE |
Additional Info
| Function | Key player in the regulation of energy balance and body weight control. Once released into the circulation, has central and peripheral effects by binding LEPR, found in many tissues, which results in the activation of several major signaling pathways. In the hypothalamus, acts as an appetite-regulating factor that induces a decrease in food intake and an increase in energy consumption by inducing anorexinogenic factors and suppressing orexigenic neuropeptides, also regulates bone mass and secretion of hypothalamo-pituitary-adrenal hormones. In the periphery, increases basal metabolism, influences reproductive function, regulates pancreatic beta-cell function and insulin secretion, is pro-angiogenic for endothelial cell and affects innate and adaptive immunity. In the arcuate nucleus of the hypothalamus, activates by depolarization POMC neurons inducing FOS and SOCS3 expression to release anorexigenic peptides and inhibits by hyperpolarization NPY neurons inducing SOCS3 with a consequent reduction on release of orexigenic peptides. In addition to its known satiety inducing effect, has a modulatory role in nutrient absorption. In the intestine, reduces glucose absorption by enterocytes by activating PKC and leading to a sequential activation of p38, PI3K and ERK signaling pathways which exerts an inhibitory effect on glucose absorption. Acts as a growth factor on certain tissues, through the activation of different signaling pathways increases expression of genes involved in cell cycle regulation such as CCND1, via JAK2-STAT3 pathway, or VEGFA, via MAPK1/3 and PI3K-AKT1 pathways. May also play an apoptotic role via JAK2-STAT3 pathway and up-regulation of BIRC5 expression. Pro-angiogenic, has mitogenic activity on vascular endothelial cells and plays a role in matrix remodeling by regulating the expression of matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs). In innate immunity, modulates the activity and function of neutrophils by increasing chemotaxis and the secretion of oxygen radicals. Increases phagocytosis by macrophages and enhances secretion of pro-inflammatory mediators. Increases cytotoxic ability of NK cells (Probable). Plays a pro-inflammatory role, in synergy with IL1B, by inducing NOS2 which promotes the production of IL6, IL8 and Prostaglandin E2, through a signaling pathway that involves JAK2, PI3K, MAP2K1/MEK1 and MAPK14/p38. In adaptive immunity, promotes the switch of memory T-cells towards T helper-1 cell immune responses. Increases CD4(+)CD25(-) T cells proliferation and reduces autophagy during TCR (T cell receptor) stimulation, through MTOR signaling pathway activation and BCL2 up-regulation. |
| Protein Name | LeptinObesity Factor |
| Database Links | Reactome: R-MMU-381771Reactome: -MMU-422085 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | Leptin proteinObesity Factor proteinLep proteinOb protein |
Information sourced from Uniprot.org