Mouse Ldlr (Low-density lipoprotein receptor) Sandwich ELISA Kit (STJE0004862)

Name: Mouse Ldlr (Low-density lipoprotein receptor) Sandwich ELISA Kit (STJE0004862)
SKU: STJE0004862
Availability: InStock

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SPECIFICATIONS

STJE0004862

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Short Description	This Ldlr Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse serum, plasma and other biological fluids.
Applications	ELISA
Reactivity	Mouse
Sensitivity	0.09ng/mL
Detection Limit	0.16~10ng/mL
Note	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Storage Instruction	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time	3.5h
Detection	Colormetric

Gene Symbol	Ldlr
Gene ID	16835
Uniprot ID	LDLR_MOUSE
Specificity	This kit recognizes Mouse LDLR in samples. No significant cross-reactivity or interference between Mouse LDLR and analogues was observed.
Sample Type	Serum, plasma and other biological fluids

Function	Binds low density lipoprotein /LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. Forms a ternary complex with PGRMC1 and TMEM97 receptors which increases LDLR-mediated LDL internalization.
Protein Name	Low-Density Lipoprotein Receptor Ldl Receptor
Database Links	Reactome: R-MMU-8856825 Reactome: -MMU-8856828 Reactome: -MMU-8964026 Reactome: -MMU-8964038
Cellular Localisation	Cell Membrane Single-Pass Type I Membrane Protein Membrane Clathrin-Coated Pit Golgi Apparatus Early Endosome Late Endosome Lysosome Rapidly Endocytosed Upon Ligand Binding
Alternative ELISA Names	Low-Density Lipoprotein Receptor ELISA kit Ldl Receptor ELISA kit Ldlr ELISA kit
Specificity	This kit recognizes Mouse LDLR in samples. No significant cross-reactivity or interference between Mouse LDLR and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (ng/mL)	0.50	0.88	4.90	0.54	0.87	4.89
Standard deviation	0.03	0.04	0.27	0.03	0.04	0.16
CV (%)	6.00	4.55	5.51	5.56	4.60	3.27