• Wild-type mouse bone marrow-derived eosinophils (n = 4) were incubated with or without DPI (20 µM) for 5 min before adding either a control mAb or Siglec-F mAb and then cells were cultured in IL-5 for an additional 18 hr before determining Annexin positivity.
  • Wild-type mouse bone marrow-derived eosinophils (n = 4) were incubated with or without DPI (20 µM) for 5 min before adding either a control mAb or Siglec-F mAb and then cells were cultured in IL-5 for an additional 18 hr before determining Annexin positivity.

Mouse Interleukin-5 protein (Recombinant) (STJP000354)

SKU:
STJP000354

£136.50 - £4,866.50
Current Stock:
Host: Insect cells
Note: STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Recombinant-Mouse Interleukin-5-protein was developed from insect cells. For use in research applications.
Conjugation: Unconjugated
Formulation: Lyophilised from 0.2 Mu m filtered PBS solution.
Dilution Range: Spin the vial and reconstite in distilled water to a concentration not less than 0.1 mg/mL. This can then be diluted into other buffers.
Storage Instruction: Can be stored in working aliquots at°C-°C C for one month, or at-20°C C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. NA
Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg (1EU/µg). NA
Immunoreactivity: The ED (50) was determined by the dose-dependent proliferation of TF-1 cells was ≤.2 ng/ml, corresponding to a specific activity of ≥ x units/mg. NA
Gene Symbol: Il5
Gene ID: 16191
Uniprot ID: IL5_MOUSE
Immunogen Region: Mature chain
Immunogen: Optimized DNA sequence encoding Mouse Interleukin-5 mature chain was expressed in Insect Cells NA
Immunogen Sequence: MRRMLLHLSV LTLSCVWATA MEIPMSTVVK ETLTQLSAHR ALLTSNETMR LPVPTHKNHQ LCIGEIFQGL DILKNQTVRG GTVEMLFQNL SLIKKYIDRQ KEKCGEERRR TRQFLDYLQE FLGVMSTEWA MEG NA
Function Homodimeric cytokine expressed predominantly by T-lymphocytes and NK cells that plays an important role in the survival, differentiation, and chemotaxis of eosinophils. Acts also on activated and resting B-cells to induce immunoglobulin production, growth, and differentiation. Mechanistically, exerts its biological effects through a receptor composed of IL5RA subunit and the cytokine receptor common subunit beta/CSF2RB. Binding to the receptor leads to activation of various kinases including LYN, SYK and JAK2 and thereby propagates signals through the RAS-MAPK and JAK-STAT5 pathways respectively.
Protein Name Interleukin-5
Il-5
B-Cell Growth Factor Ii
Bcgf-Ii
Cytotoxic T-Lymphocyte Inducer
Eosinophil Differentiation Factor
T-Cell Replacing Factor
Trf
Cellular Localisation Secreted
Alternative Protein Names Interleukin-5 protein
Il-5 protein
B-Cell Growth Factor Ii protein
Bcgf-Ii protein
Cytotoxic T-Lymphocyte Inducer protein
Eosinophil Differentiation Factor protein
T-Cell Replacing Factor protein
Trf protein
Il5 protein
Il-5 protein

Information sourced from Uniprot.org

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