Mouse Interleukin-5 protein (Recombinant) (STJP000354)
SPECIFICATIONS
HostInsect cells
ConjugationUnconjugated
ImmunogenOptimized DNA sequence encoding Mouse Interleukin-5 mature chain was expressed in Insect Cells NA
General Information
| Short Description | Recombinant-Mouse Interleukin-5-protein was developed from insect cells. For use in research applications. |
| Host | Insect cells |
| Note | STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Dilution Range | Spin the vial and reconstite in distilled water to a concentration not less than 0.1 mg/mL. This can then be diluted into other buffers. |
| Formulation | Lyophilised from 0.2 Mu m filtered PBS solution. |
| Storage Instruction | Can be stored in working aliquots at°C-°C C for one month, or at-20°C C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. NA |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg (1EU/µg). NA |
| Immunoreactivity | The ED (50) was determined by the dose-dependent proliferation of TF-1 cells was ≤.2 ng/ml, corresponding to a specific activity of ≥ x units/mg. NA |
Target Information
| Gene Symbol | Il5 |
| Gene ID | 16191 |
| Uniprot ID | IL5_MOUSE |
| Immunogen | Optimized DNA sequence encoding Mouse Interleukin-5 mature chain was expressed in Insect Cells NA |
| Immunogen Region | Mature chain |
| Immunogen Sequence | MRRMLLHLSV LTLSCVWATA MEIPMSTVVK ETLTQLSAHR ALLTSNETMR LPVPTHKNHQ LCIGEIFQGL DILKNQTVRG GTVEMLFQNL SLIKKYIDRQ KEKCGEERRR TRQFLDYLQE FLGVMSTEWA MEG NA |
Additional Info
| Tissue Specificity | Expressed in lymphoid cells, including spleen, thymus, lymph nodes and peripheral blood mononuclear cells. |
| Function | Homodimeric cytokine expressed predominantly by T-lymphocytes and NK cells that plays an important role in the survival, differentiation, and chemotaxis of eosinophils. Also acts on activated and resting B-cells to induce immunoglobulin production, growth, and differentiation. Mechanistically, exerts its biological effects through a receptor composed of IL5RA subunit and the cytokine receptor common subunit beta/CSF2RB. Binding to the receptor leads to activation of various kinases including LYN, SYK and JAK2 and thereby propagates signals through the RAS-MAPK and JAK-STAT5 pathways respectively. |
| Protein Name | Interleukin-5Il-5B-Cell Growth Factor IiBcgf-IiCytotoxic T-Lymphocyte InducerEosinophil Differentiation FactorT-Cell Replacing FactorTrf |
| Database Links | Reactome: R-MMU-512988Reactome: -MMU-5673001Reactome: -MMU-912526 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | Interleukin-5 proteinIl-5 proteinB-Cell Growth Factor Ii proteinBcgf-Ii proteinCytotoxic T-Lymphocyte Inducer proteinEosinophil Differentiation Factor proteinT-Cell Replacing Factor proteinTrf proteinIl5 proteinIl-5 protein |
Information sourced from Uniprot.org