Mouse IL3 protein (Recombinant-Active) (C-His) (STJP011778)
SPECIFICATIONS
HostMammalian Cells
ConjugationUnconjugated
ImmunogenMus musculus (Mouse)
General Information
| Short Description | Recombinant-Active-Mouse IL3-C-His protein was developed from mammalian cells and has a target region of C-His. For use in research applications. |
| Applications | ELISA/WB |
| Host | Mammalian Cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Dilution Range | Reconstitute in sterile water for a stock solution. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 5% Trehalose, 5% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at-20 to-80°C for twelve months from the date of receipt. |
| Endotoxin | <0.1 EU/Mu g of the protein by the LAL method. |
| Immunoreactivity | Measured in a cell proliferation assay using mouse pro-B cells (BaF3). The ED50 for this effect is 1.5-6 pg/mL, corresponding to a specific activity of 1.67 * 10^8-6.67* 10^8 units/mg. |
Target Information
| Gene Symbol | Il3 |
| Gene ID | 16187 |
| Uniprot ID | IL3_MOUSE |
| Immunogen | Mus musculus (Mouse) |
| Immunogen Region | Met1-Cys166 |
Additional Info
| Function | Cytokine secreted predominantly by activated T-lymphocytes as well as mast cells and osteoblastic cells that controls the production and differentiation of hematopoietic progenitor cells into lineage-restricted cells. Also stimulates mature basophils, eosinophils, and monocytes to become functionally activated. In addition, plays an important role in neural cell proliferation and survival. Participates as well in bone homeostasis and inhibits osteoclast differentiation by preventing NF-kappa-B nuclear translocation and activation. Mechanistically, exerts its biological effects through a receptor composed of IL3RA subunit and a signal transducing subunit IL3RB. Receptor stimulation results in the rapid activation of JAK2 kinase activity leading to STAT5-mediated transcriptional program. Alternatively, contributes to cell survival under oxidative stress in non-hematopoietic systems by activating pathways mediated by PI3K/AKT and ERK. |
| Protein Name | Interleukin-3Il-3Hematopoietic Growth FactorMast Cell Growth FactorMcgfMultipotential Colony-Stimulating FactorP-Cell-Stimulating Factor |
| Database Links | Reactome: R-MMU-512988Reactome: -MMU-5673001Reactome: -MMU-912526 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | Interleukin-3 proteinIl-3 proteinHematopoietic Growth Factor proteinMast Cell Growth Factor proteinMcgf proteinMultipotential Colony-Stimulating Factor proteinP-Cell-Stimulating Factor proteinIl3 proteinCsfmu proteinIl-3 protein |
Information sourced from Uniprot.org