| Host: | HEK293 cells |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Formulation: | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method: | <0.1 EU/Mu g |
| Immunoreactivity: | Measured in a cell proliferation assay using TF-1 Mouse erythroleukemic cells. The ED50 for this effect is 0.12-0.49ng/mL. |
| Gene Symbol: | Il5 |
| Gene ID: | 16191 |
| Uniprot ID: | IL5_MOUSE |
| Immunogen Region: | Met21-Gly133 |
| Immunogen: | Recombinant Mouse IL-5 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met21-Gly133) of mouse IL-5 (Accession #NP_034688.1) fused with and a 6×His tag at the C-terminus. |
| Immunogen Sequence: | MEIPMSTVVKETLTQLSAHR ALLTSNETMRLPVPTHKNHQ LCIGEIFQGLDILKNQTVRG GTVEMLFQNLSLIKKYIDRQ KEKCGEERRRTRQFLDYLQE FLGVMSTEWAMEG |
| Function | Homodimeric cytokine expressed predominantly by T-lymphocytes and NK cells that plays an important role in the survival, differentiation, and chemotaxis of eosinophils. Acts also on activated and resting B-cells to induce immunoglobulin production, growth, and differentiation. Mechanistically, exerts its biological effects through a receptor composed of IL5RA subunit and the cytokine receptor common subunit beta/CSF2RB. Binding to the receptor leads to activation of various kinases including LYN, SYK and JAK2 and thereby propagates signals through the RAS-MAPK and JAK-STAT5 pathways respectively. |
| Protein Name | Interleukin-5 Il-5 B-Cell Growth Factor Ii Bcgf-Ii Cytotoxic T-Lymphocyte Inducer Eosinophil Differentiation Factor T-Cell Replacing Factor Trf |
| Database Links | Reactome: R-MMU-512988 Reactome: -MMU-5673001 Reactome: -MMU-912526 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | Interleukin-5 protein Il-5 protein B-Cell Growth Factor Ii protein Bcgf-Ii protein Cytotoxic T-Lymphocyte Inducer protein Eosinophil Differentiation Factor protein T-Cell Replacing Factor protein Trf protein Il5 protein Il-5 protein |
Information sourced from Uniprot.org