Mouse IL-5 protein (Recombinant) (C-6His) (STJP002874)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Mouse IL-5 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met21-Gly133) of mouse IL-5 (Accession #NP_034688.1) fused with and a 6×His tag at the C-terminus.
General Information
| Short Description | Recombinant-Mouse IL-5-C-6His protein was developed in hek293 cells using the region Met21-Gly133. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | Measured in a cell proliferation assay using TF-1 Mouse erythroleukemic cells. The ED50 for this effect is 0.12-0.49ng/mL. |
| Determination Method | <0.1 EU/Mu g |
Target Information
| Gene Symbol | Il5 |
| Gene ID | 16191 |
| Uniprot ID | IL5_MOUSE |
| Immunogen | Recombinant Mouse IL-5 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met21-Gly133) of mouse IL-5 (Accession #NP_034688.1) fused with and a 6×His tag at the C-terminus. |
| Immunogen Region | Met21-Gly133 |
| Immunogen Sequence | MEIPMSTVVKETLTQLSAHR ALLTSNETMRLPVPTHKNHQ LCIGEIFQGLDILKNQTVRG GTVEMLFQNLSLIKKYIDRQ KEKCGEERRRTRQFLDYLQE FLGVMSTEWAMEG |
Additional Info
| Function | Homodimeric cytokine expressed predominantly by T-lymphocytes and NK cells that plays an important role in the survival, differentiation, and chemotaxis of eosinophils. Also acts on activated and resting B-cells to induce immunoglobulin production, growth, and differentiation. Mechanistically, exerts its biological effects through a receptor composed of IL5RA subunit and the cytokine receptor common subunit beta/CSF2RB. Binding to the receptor leads to activation of various kinases including LYN, SYK and JAK2 and thereby propagates signals through the RAS-MAPK and JAK-STAT5 pathways respectively. |
| Protein Name | Interleukin-5Il-5B-Cell Growth Factor IiBcgf-IiCytotoxic T-Lymphocyte InducerEosinophil Differentiation FactorT-Cell Replacing FactorTrf |
| Database Links | Reactome: R-MMU-512988Reactome: -MMU-5673001Reactome: -MMU-912526 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | Interleukin-5 proteinIl-5 proteinB-Cell Growth Factor Ii proteinBcgf-Ii proteinCytotoxic T-Lymphocyte Inducer proteinEosinophil Differentiation Factor proteinT-Cell Replacing Factor proteinTrf proteinIl5 proteinIl-5 protein |
Information sourced from Uniprot.org