Mouse IL-5 (Interleukin-5) Antibody Pair (STJAP00537)
General Information
| Short Description | This IL-5 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Rat/Rat |
| Reactivity | Mouse |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Protein A or G.Detection Purification: Protein A or G. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300; 50% glycerol; pH 7.5.Detection Buffer: PBS with 0.04% Proclin 300; 1% protective protein; 50% glycerol; pH 7.5. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
| Determination Method | Colormetric |
Target Information
| Gene Symbol | Il5 |
| Gene ID | 16191 |
| Uniprot ID | IL5_MOUSE |
| Immunogen | Capture immunogen: Recombinant Mouse IL-5 protien.Detection Immunogen: Recombinant Mouse IL-5 protien. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Function | Homodimeric cytokine expressed predominantly by T-lymphocytes and NK cells that plays an important role in the survival, differentiation, and chemotaxis of eosinophils. Also acts on activated and resting B-cells to induce immunoglobulin production, growth, and differentiation. Mechanistically, exerts its biological effects through a receptor composed of IL5RA subunit and the cytokine receptor common subunit beta/CSF2RB. Binding to the receptor leads to activation of various kinases including LYN, SYK and JAK2 and thereby propagates signals through the RAS-MAPK and JAK-STAT5 pathways respectively. |
| Protein Name | Interleukin-5Il-5B-Cell Growth Factor IiBcgf-IiCytotoxic T-Lymphocyte InducerEosinophil Differentiation FactorT-Cell Replacing FactorTrf |
| Database Links | Reactome: R-MMU-512988Reactome: -MMU-5673001Reactome: -MMU-912526 |
| Cellular Localisation | Secreted |
| Alternative Antibody Pair Names | Interleukin-5 antibody pairIl-5 antibody pairB-Cell Growth Factor Ii antibody pairBcgf-Ii antibody pairCytotoxic T-Lymphocyte Inducer antibody pairEosinophil Differentiation Factor antibody pairT-Cell Replacing Factor antibody pairTrf antibody pairIl5 antibody pairIl-5 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Mouse IL-5 (Interleukin-5) Antibody Pair (STJAP00537)
This antibody pair detects mouse Interleukin-5 (IL-5), corresponding to UniProt accession P04401. Reactivity has been confirmed for mouse samples only.
The capture antibody is unconjugated and the detection antibody is biotin-conjugated. Both are supplied at 0.5 mg/mL, are rat-derived, and have been purified by Protein A or G.
This pair has been validated for use in ELISA. No other assay formats are listed in the product specifications.
Serum, plasma, and other biological fluids are listed as compatible sample types for use with this pair in ELISA-based detection of mouse IL-5.
The capture antibody is formulated in PBS with 0.04% Proclin 300 and 50% glycerol at pH 7.5. The detection antibody is formulated in PBS with 0.04% Proclin 300, 1% protective protein, and 50% glycerol at pH 7.5. Both components should be stored at -20 degrees Celsius for long-term storage, and freeze-thaw cycles should be avoided.
Both the capture and detection antibodies were raised against recombinant mouse IL-5 protein. Both are rat-derived and purified by Protein A or G affinity chromatography.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.