Mouse IL-5 (IL-5) Antibody Pair (STJAP00744)
General Information
| Short Description | This IL-5 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the. |
| Applications | ELISA |
| Host | Rabbit/Rabbit |
| Reactivity | Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Monoclonal |
| Clone ID | R04-5P8/R03-6T5 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | Determined by end user |
| Formulation | 0.01M PBS, pH 7.2 |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | Il5 |
| Gene ID | 16191 |
| Uniprot ID | IL5_MOUSE |
| Immunogen | Recombinant protein |
Additional Info
| Tissue Specificity | Expressed in lymphoid cells, including spleen, thymus, lymph nodes and peripheral blood mononuclear cells. |
| Function | Homodimeric cytokine expressed predominantly by T-lymphocytes and NK cells that plays an important role in the survival, differentiation, and chemotaxis of eosinophils. Also acts on activated and resting B-cells to induce immunoglobulin production, growth, and differentiation. Mechanistically, exerts its biological effects through a receptor composed of IL5RA subunit and the cytokine receptor common subunit beta/CSF2RB. Binding to the receptor leads to activation of various kinases including LYN, SYK and JAK2 and thereby propagates signals through the RAS-MAPK and JAK-STAT5 pathways respectively. |
| Protein Name | Interleukin-5Il-5B-Cell Growth Factor IiBcgf-IiCytotoxic T-Lymphocyte InducerEosinophil Differentiation FactorT-Cell Replacing FactorTrf |
| Database Links | Reactome: R-MMU-512988Reactome: -MMU-5673001Reactome: -MMU-912526 |
| Cellular Localisation | Secreted |
| Alternative Antibody Pair Names | Interleukin-5 antibody pairIl-5 antibody pairB-Cell Growth Factor Ii antibody pairBcgf-Ii antibody pairCytotoxic T-Lymphocyte Inducer antibody pairEosinophil Differentiation Factor antibody pairT-Cell Replacing Factor antibody pairTrf antibody pairIl5 antibody pairIl-5 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Mouse IL-5 (IL-5) Antibody Pair (STJAP00744)
This antibody pair is designed to detect mouse IL-5 (Interleukin-5), with reactivity confirmed for mouse. The target protein corresponds to UniProt accession P04401.
Both antibodies are rabbit monoclonal IgGs supplied unconjugated at 1 mg/mL, formulated in 0.01M PBS at pH 7.2, and purified by affinity chromatography. The clone IDs are R04-5P8 and R03-6T5 respectively.
This pair has been validated for use in ELISA. No other assay formats are listed in the product specifications.
Working dilutions for both the capture and detection antibodies are determined by the end user and are not defined in the product specifications. Titration experiments are recommended to establish optimal concentrations for each specific assay setup.
Both antibodies should be stored at 4°C for short-term use. For long-term storage, aliquot and keep at -20°C. Repeated freeze/thaw cycles should be avoided to maintain activity.
Both antibodies were raised against a recombinant protein immunogen corresponding to mouse IL-5. No further detail on the specific immunogen region is provided in the product specifications.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.