Mouse IL-4 (IL-4) Antibody Pair (STJAP00743)
General Information
| Short Description | This IL-4 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the. |
| Applications | ELISA |
| Host | Rabbit/Rabbit |
| Reactivity | Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Monoclonal |
| Clone ID | R07-2T9/R04-8O2 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | Determined by end user |
| Formulation | 0.01M PBS, pH 7.2 |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | Il4 |
| Gene ID | 16189 |
| Uniprot ID | IL4_MOUSE |
| Immunogen | Recombinant protein |
Additional Info
| Function | Cytokine secreted primarily by mast cells, T-cells, eosinophils, and basophils that plays a role in regulating antibody production, hematopoiesis and inflammation, and the development of effector T-cell responses. Induces the expression of class II MHC molecules on resting B-cells. Enhances both secretion and cell surface expression of IgE and IgG1. Also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4. In addition, plays a critical role in higher functions of the normal brain, such as memory and learning. Upon binding to IL4, IL4R receptor dimerizes either with the common IL2R gamma chain/IL2RG to produce the type 1 signaling complex, located mainly on hematopoietic cells, or with the IL13RA1 to produce the type 2 complex, which is also expressed on nonhematopoietic cells. Engagement of both types of receptors initiates JAK3 and to a lower extend JAK1 phosphorylation leading to activation of the signal transducer and activator of transcription 6/STAT6. |
| Protein Name | Interleukin-4Il-4B-Cell Igg Differentiation FactorB-Cell Growth Factor 1B-Cell Stimulatory Factor 1Bsf-1Igg1 Induction FactorLymphocyte Stimulatory Factor 1 |
| Database Links | Reactome: R-MMU-6785807 |
| Cellular Localisation | Secreted |
| Alternative Antibody Pair Names | Interleukin-4 antibody pairIl-4 antibody pairB-Cell Igg Differentiation Factor antibody pairB-Cell Growth Factor 1 antibody pairB-Cell Stimulatory Factor 1 antibody pairBsf-1 antibody pairIgg1 Induction Factor antibody pairLymphocyte Stimulatory Factor 1 antibody pairIl4 antibody pairIl-4 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Mouse IL-4 (IL-4) Antibody Pair (STJAP00743)
This antibody pair is designed to detect mouse IL-4, corresponding to UniProt accession P07750. Reactivity is confirmed for mouse samples only.
This antibody pair has been validated for use in ELISA. No other assay formats are listed for this product.
Both antibodies are rabbit monoclonal IgGs supplied unconjugated at 1 mg/mL, formulated in 0.01M PBS at pH 7.2, and purified by affinity chromatography. Clone IDs are R07-2T9 and R04-8O2 respectively.
Optimal working concentrations are not pre-defined for this pair and must be determined by the end user through titration experiments.
Both components should be stored at 4°C for short-term use. For long-term storage, aliquot and keep at -20°C. Repeated freeze/thaw cycles should be avoided to maintain activity.
Both antibodies in this pair were raised against a recombinant protein immunogen corresponding to mouse IL-4. No further detail on the immunogen region is provided in the product specification.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.