Mouse IL-2 (IL-2) Antibody Pair (STJAP00742)
General Information
| Short Description | This IL-2 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the. |
| Applications | ELISA |
| Host | Rabbit/Rabbit |
| Reactivity | Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Monoclonal |
| Clone ID | R06-8X4/R03-6F5 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | Determined by end user |
| Formulation | 0.01M PBS, pH 7.2 |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | Il2 |
| Gene ID | 16183 |
| Uniprot ID | IL2_MOUSE |
| Immunogen | Recombinant protein |
Additional Info
| Tissue Specificity | Produced by immune cells including dendritic cells. In contrast, macrophages do not produce IL2 upon bacterial stimulation. |
| Function | Cytokine produced by activated CD4-positive helper T-cells and to a lesser extend activated CD8-positive T-cells and natural killer (NK) cells that plays pivotal roles in the immune response and tolerance. Binds to a receptor complex composed of either the high-affinity trimeric IL-2R (IL2RA/CD25, IL2RB/CD122 and IL2RG/CD132) or the low-affinity dimeric IL-2R (IL2RB and IL2RG). Interaction with the receptor leads to oligomerization and conformation changes in the IL-2R subunits resulting in downstream signaling starting with phosphorylation of JAK1 and JAK3. In turn, JAK1 and JAK3 phosphorylate the receptor to form a docking site leading to the phosphorylation of several substrates including STAT5. This process leads to activation of several pathways including STAT, phosphoinositide-3-kinase/PI3K and mitogen-activated protein kinase/MAPK pathways. Functions as a T-cell growth factor and can increase NK-cell cytolytic activity as well. Promotes strong proliferation of activated B-cells and subsequently immunoglobulin production. Plays a pivotal role in regulating the adaptive immune system by controlling the survival and proliferation of regulatory T-cells, which are required for the maintenance of immune tolerance. Moreover, participates in the differentiation and homeostasis of effector T-cell subsets, including Th1, Th2, Th17 as well as memory CD8-positive T-cells. |
| Protein Name | Interleukin-2Il-2T-Cell Growth FactorTcgf |
| Database Links | Reactome: R-MMU-5673001Reactome: -MMU-9020558Reactome: -MMU-912526 |
| Cellular Localisation | Secreted |
| Alternative Antibody Pair Names | Interleukin-2 antibody pairIl-2 antibody pairT-Cell Growth Factor antibody pairTcgf antibody pairIl2 antibody pairIl-2 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Mouse IL-2 (IL-2) Antibody Pair (STJAP00742)
This antibody pair is designed to detect mouse IL-2 (Interleukin-2), with reactivity confirmed for mouse. The target protein corresponds to UniProt accession P04351.
This antibody pair has been validated for use in ELISA. It is intended for research use only and is not validated for diagnostic or therapeutic applications.
Both antibodies are rabbit monoclonal IgGs supplied unconjugated at 1 mg/mL in 0.01M PBS, pH 7.2. Clone IDs are R06-8X4 and R03-6F5, and both have been affinity purified.
The optimal working dilutions for both antibodies are to be determined by the end user. Titration experiments are recommended to establish the appropriate concentrations for each specific assay setup.
Both antibodies should be stored at 4°C for short-term use. For long-term storage, aliquot and store at -20°C, and avoid repeated freeze/thaw cycles to maintain activity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.