Mouse IL-1ra/IL-1F3 (Interleukin 1 Receptor Antagonist) Sandwich ELISA Kit (STJE0019986)
SPECIFICATIONS
General Information
| Short Description | This IL-1ra/IL-1F3 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse serum, plasma and other biological fluids. |
| Applications | ELISA |
| Reactivity | Mouse |
| Sensitivity | 15.75 pg/mL |
| Detection Limit | 31.25-2000 pg/mL |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Storage Instruction | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time | 3.5h |
| Detection | Colormetric |
Target Information
| Gene Symbol | Il1rn |
| Gene ID | 16181 |
| Uniprot ID | IL1RA_MOUSE |
| Specificity | This kit recognizes Mouse IL-1ra/IL-1F3 in samples. No significant cross-reactivity or interference between Mouse IL-1ra/IL-1F3 and analogues was observed. |
| Sample Type | serum, plasma and other biological fluids |
Additional Info
| Function | Anti-inflammatory antagonist of interleukin-1 family of proinflammatory cytokines such as interleukin-1beta/IL1B and interleukin-1alpha/IL1A. Protects from immune dysregulation and uncontrolled systemic inflammation triggered by IL1 for a range of innate stimulatory agents such as pathogens. |
| Protein Name | Interleukin-1 Receptor Antagonist ProteinIl-1rnIl-1raIrapIl1 Inhibitor |
| Database Links | Reactome: R-MMU-9020702 |
| Cellular Localisation | Isoform 1: SecretedIsoform 2: Cytoplasm |
| Alternative ELISA Names | Interleukin-1 Receptor Antagonist Protein ELISA kitIl-1rn ELISA kitIl-1ra ELISA kitIrap ELISA kitIl1 Inhibitor ELISA kitIl1rn ELISA kitIl-1ra ELISA kit |
| Specificity | This kit recognizes Mouse IL-1ra/IL-1F3 in samples. No significant cross-reactivity or interference between Mouse IL-1ra/IL-1F3 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (50×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (50×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 92-104 | 97 |
| EDTA plasma (n=8) | 92-109 | 99 |
| Cell culture media (n=8) | 95-107 | 101 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 107.81 | 298.31 | 706.86 | 106.60 | 270.20 | 704.32 |
| Standard deviation | 5.46 | 17.78 | 21.84 | 6.12 | 11.65 | 29.58 |
| CV (%) | 5.06 | 5.96 | 3.09 | 5.74 | 4.31 | 4.20 |