Mouse IL-18 (Interleukin-18) Antibody Pair (STJAP00538)
General Information
| Short Description | This IL-18 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Rat/Rabbit |
| Reactivity | Mouse |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Protein A or G.Detection Purification: Protein A or G. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300; 50% glycerol; pH 7.5.Detection Buffer: PBS with 0.04% Proclin 300; 1% protective protein; 50% glycerol; pH 7.5. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
| Determination Method | Colormetric |
Target Information
| Gene Symbol | Il18 |
| Gene ID | 16173 |
| Uniprot ID | IL18_MOUSE |
| Immunogen | Capture immunogen: Recombinant Mouse IL-18 protien.Detection Immunogen: Recombinant Mouse IL-18 protien. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Function | Pro-inflammatory cytokine primarily involved in epithelial barrier repair, polarized T-helper 1 (Th1) cell and natural killer (NK) cell immune responses. Upon binding to IL18R1 and IL18RAP, forms a signaling ternary complex which activates NF-kappa-B, triggering synthesis of inflammatory mediators. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells and natural killer (NK) cells. Involved in transduction of inflammation downstream of pyroptosis: its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore. |
| Protein Name | Interleukin-18Il-18Interferon Gamma-Inducing FactorIfn-Gamma-Inducing FactorInterleukin-1 GammaIl-1 Gamma |
| Database Links | Reactome: R-MMU-448706Reactome: -MMU-5620971Reactome: -MMU-9012546 |
| Cellular Localisation | CytoplasmSecretedThe Precursor Is CytosolicIn Response To Inflammasome-Activating SignalsCleaved And SecretedMature Form Is Secreted And Released In The Extracellular Milieu By Passing Through The Gasdermin-D (Gsdmd) PoreIn ContrastThe Precursor Form Is Not ReleasedDue To The Presence Of An Acidic Region That Is Proteolytically Removed By Casp1 During MaturationThe Secretion Is Dependent On Protein Unfolding And Facilitated By The Cargo Receptor Tmed10 |
| Alternative Antibody Pair Names | Interleukin-18 antibody pairIl-18 antibody pairInterferon Gamma-Inducing Factor antibody pairIfn-Gamma-Inducing Factor antibody pairInterleukin-1 Gamma antibody pairIl-1 Gamma antibody pairIl18 antibody pairIgif antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Mouse IL-18 (Interleukin-18) Antibody Pair (STJAP00538)
This antibody pair detects mouse Interleukin-18 (IL-18), corresponding to UniProt entry P70380, and has confirmed reactivity with mouse samples. It is not validated for IL-18 detection in other species.
The capture antibody is unconjugated and the detection antibody is biotin-conjugated, with both components supplied at 0.5 mg/mL. Both antibodies were raised using recombinant mouse IL-18 protein as immunogen and were purified by Protein A or G affinity chromatography.
This pair is validated for ELISA only. No other assay formats are listed as supported applications.
This antibody pair is suitable for use with mouse serum, plasma, and other biological fluids. Compatibility with additional sample types such as cell culture supernatants has not been specified in the product details.
The capture antibody is formulated in PBS with 0.04% Proclin 300 and 50% glycerol at pH 7.5, while the detection antibody is formulated in PBS with 0.04% Proclin 300, 1% protective protein, and 50% glycerol at pH 7.5. Both components should be stored at -20°C for long-term storage, and freeze-thaw cycles should be avoided.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.