Mouse ICAM-1/CD54 (Intercellular Adhesion Molecule 1) Antibody Pair (STJAP00528)
General Information
| Short Description | This ICAM-1/CD54 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Rat/Goat |
| Reactivity | Mouse |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Protein A or G.Detection Purification: Antigen Affinity. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300; 50% glycerol; pH 7.5.Detection Buffer: PBS with 0.04% Proclin 300; 1% protective protein; 50% glycerol; pH 7.5. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
| Determination Method | Colormetric |
Target Information
| Gene Symbol | Icam1 |
| Gene ID | 15894 |
| Uniprot ID | ICAM1_MOUSE |
| Immunogen | Capture immunogen: Recombinant Mouse ICAM-1/CD54 protien.Detection Immunogen: Recombinant Mouse ICAM-1/CD54 protien. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Post Translational Modifications | Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis. |
| Function | ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. |
| Protein Name | Intercellular Adhesion Molecule 1Icam-1Mala-2Myd10Cd Antigen Cd54 |
| Database Links | Reactome: R-MMU-198933Reactome: -MMU-216083 |
| Cellular Localisation | MembraneSingle-Pass Type I Membrane Protein |
| Alternative Antibody Pair Names | Intercellular Adhesion Molecule 1 antibody pairIcam-1 antibody pairMala-2 antibody pairMyd10 antibody pairCd Antigen Cd54 antibody pairIcam1 antibody pairIcam-1 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Mouse ICAM-1/CD54 (Intercellular Adhesion Molecule 1) Antibody Pair (STJAP00528)
This antibody pair is designed to detect mouse ICAM-1/CD54 (Intercellular Adhesion Molecule 1), with reactivity confirmed for mouse samples. It is intended for use with serum, plasma, and other biological fluids.
The capture antibody is unconjugated and supplied at 0.5 mg/mL in PBS with 0.04% Proclin 300 and 50% glycerol at pH 7.5. The detection antibody is biotin-conjugated and supplied at 0.5 mg/mL in PBS with 0.04% Proclin 300, 1% protective protein, and 50% glycerol at pH 7.5.
This pair has been validated for use in ELISA. It is intended for sandwich ELISA-based detection of mouse ICAM-1/CD54 in research samples.
The capture antibody was raised in rat and purified by Protein A or G, while the detection antibody was raised in goat and purified by antigen affinity. Both were generated against recombinant mouse ICAM-1/CD54 protein.
Both components should be stored at -20°C for long-term storage. Repeated freeze-thaw cycles should be avoided to maintain reagent integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.