This HSD3b1 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids..
Applications
ELISA
Reactivity
Mouse
Sensitivity
29.3pg/mL
Detection Limit
78.1-5000pg/mL
Note
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Product Properties
Storage Instruction
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Additional Info
Tissue Specificity
Steroidogenic tissues (includes testes, ovaries and adrenal glands).
Post Translational Modifications
Function
A bifunctional enzyme responsible for the oxidation and isomerization of 3beta-hydroxy-Delta(5)-steroid precursors to 3-oxo-Delta(4)-steroids, an essential step in steroid hormone biosynthesis. Specifically catalyzes the conversion of pregnenolone to progesterone, 17alpha-hydroxypregnenolone to 17alpha-hydroxyprogesterone, dehydroepiandrosterone (DHEA) to 4-androstenedione, and androstenediol to testosterone. Additionally, catalyzes the interconversion between 3beta-hydroxy and 3-oxo-5alpha-androstane steroids controlling the bioavalability of the active forms. Specifically converts dihydrotestosterone to its inactive form 5alpha-androstanediol, that does not bind androgen receptor/AR. Also converts androstanedione, a precursor of testosterone and estrone, to epiandrosterone. Expected to use NAD(+) as preferred electron donor for the 3-beta-hydroxy-steroid dehydrogenase activity and NADPH for the 3-ketosteroid reductase activity.
Protein Name
3 Beta-Hydroxysteroid Dehydrogenase/Delta 5-->4-Isomerase Type 13 Beta-Hydroxysteroid Dehydrogenase/Delta 5-->4-Isomerase Type I3-Beta-Hsd I3-Beta-Hydroxy-5-Ene Steroid Dehydrogenase3-Beta-Hydroxy-Delta(5-Steroid Dehydrogenase3-Beta-Hydroxysteroid 3-DehydrogenaseDelta-5-3-Ketosteroid IsomeraseDihydrotestosterone OxidoreductaseSteroid Delta-Isomerase
