• BxPC-3 or ASPC-1 cells infected with recombinant lentivirus expressing Met knockdown shRNAs (2 or 5) or a non targeting shRNA were treated without or with HGF and examined by Western analysis for pMet (Y1234/1235) , Met, pErk1/2, Erk1/2, pAKT, AKT and Beta-actin levels (n = 3).
  • Hepatic. Cells were seeded at a concentration of 5,000 cells/cm2 on tissue culture plastic plates and coverslips coated with Matrigel and cultured in high glucose DMEM supplemented with 1% Penicillin/Streptomycin, 2 mmol/l L-Glutamine and 10% FBS for 3 days. The media were then changed to high glucose DMEM supplemented with 15% FBS, 1% Penicillin/Streptomycin, 2 mmol/l L-Glutamine, 300 µmol/l Monothioglycerol, 20 ng/ml Hepatocyte Growth Factor, 10 ng/ml Oncostatin M, 10−7 Dexamethasone, 100 ng/ml FGF4, and 1X ITS. The cells were allowed to differentiate for 21 days and then fixed and stored in PBS for immunofluorescence. The differentiation media were collected and analyzed for the presence of urea secreted by the differentiated cells. Urea was subsequently measured using the Urea/Ammonia determination kit (R-Biopharm AG, Darmstadt, Germany) according to the manufacturers NA instructions. Cells were subsquently assessed for expression of the hepatocyte markers ALBUMIN and…
  • Prior to starting the hepatic differentiation, medium NA >MSCs at passage 5 were maintained in the regular medium NA >MSC culture medium until at 80–90% confluence. The hepatic differentiation was elicited in the differentiation-inducing medium, which consisted of MEM supplemented with 10% FBS, 10 ng/mL hepatocyte growth factor (HGF) , 10 ng/mL basic fibroblast growth factor (bFGF) and 10 ng/mL oncostatin M (&system). The medium was changed every 3 days, and the cells were cultured for 8 days.
  • >Stage 3: Differentiation of Premature Hepatocyte-Like Cells (5–11). At day 5, the medium was replaced by Knockout DMEM supplemented with 1% KSR, 1% nonessential amino acids, 1% l-glutamic acid, 1% dimethyl sulfoxide (DMSO) , and 100 ng/ml hepatocyte growth factor (HGF) to induce premature hepatocyte-like cells. The medium was replaced every two days.

Mouse Hepatocyte Growth Factor protein (Recombinant) (STJP000596)

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STJP000596

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Host: CHO cells
Note: STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Recombinant-Mouse Hepatocyte Growth Factor-protein was developed from cho cells. For use in research applications.
Conjugation: Unconjugated
Formulation: Lyophilised from 0.2 Mu m filtered solution in 2.5% glycine, 0.5% sucrose, 0.01% Tween80, 5 mM Glutamic acid, pH 4.5.
Dilution Range: Spin the vial and reconstite in distilled water to a concentration not less than 0.1 mg/mL. This can then be diluted into other buffers.
Storage Instruction: Can be stored in working aliquots at 2°C-8°C C for one month, or at-20°C C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. NA
Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). NA
Immunoreactivity: The ED (50) was determined by the dose-dependent stimulation of the proliferation ofmurine BNL-CL2cell li was found to be in the range of 20.0-40.0 ng/ml. NA
Gene Symbol: Hgf
Gene ID: 15234
Uniprot ID: HGF_MOUSE
Immunogen Region: Mature chain
Immunogen: Optimized DNA sequence encoding Mouse Hepatocyte Growth Factor mature chain was expressed in Chinese Hamster Ovary cell line. NA
Immunogen Sequence: MMWGTKLLPV LLLQHVLLHL LLLHVAIPYA EGQKKRRNTL HEFKKSAKTT 50 LTKEDPLLKI KTKKVNSADE CANRCIRNRG FTFTCKAFVF DKSRKRCYWY 100 PFNSMSSGVK KGFGHEFDLY ENKDYIRNCI IGKGGSYKGT VSITKSGIKC 150 QPWNSMIPHE HSFLPSSYRG KDLQENYCRN PRGEEGGPWC FTSNPEVRYE 200 VCDIPQCSEV ECMT
Post Translational Modifications The single-chain precursor undergoes proteolytic processing by TMPRSS13 resulting in an active two-chain form.
Function Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization. Activates MAPK signaling following TMPRSS13 cleavage and activation.
Protein Name Hepatocyte Growth Factor
Hepatopoietin-A
Scatter Factor
Sf Cleaved Into - Hepatocyte Growth Factor Alpha Chain - Hepatocyte Growth Factor Beta Chain
Database Links Reactome: R-MMU-114608
Reactome: -MMU-1257604
Reactome: -MMU-5673001
Reactome: -MMU-6806942
Reactome: -MMU-6807004
Reactome: -MMU-6811558
Reactome: -MMU-8851805
Reactome: -MMU-8851907
Reactome: -MMU-8865999
Reactome: -MMU-8874081
Reactome: -MMU-8875513
Reactome: -MMU-8875555
Reactome: -MMU-8875656
Reactome: -MMU-8875791
Reactome: -MMU-9734091
Alternative Protein Names Hepatocyte Growth Factor protein
Hepatopoietin-A protein
Scatter Factor protein
Sf Cleaved Into - Hepatocyte Growth Factor Alpha Chain - Hepatocyte Growth Factor Beta Chain protein
Hgf protein

Information sourced from Uniprot.org

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