This GRIA1 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse serum, plasma, tissue homogenates or other biological fluids..
Applications
ELISA
Reactivity
Mouse
Sensitivity
0.051ng/mL
Detection Limit
0.156-10ng/mL
Note
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Product Properties
Storage Instruction
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
serum, plasma, tissue homogenates or other biological fluids.
Additional Info
Tissue Specificity
Expressed in the outer plexiform layer of the retina of the eye (at protein level). Expressed in the forebrain and hippocampus (at protein level).
Post Translational Modifications
Phosphorylated at Ser-645. Phosphorylated at Ser-710 by PKC. Phosphorylated at Ser-849 by PKC, PKA and CAMK2. Phosphorylated at Ser-863 by PKC, PKA and PRKG2. Phosphorylation of Ser-863 is reduced by induction of long-term depression and increased by induction of long-term potentiation. Palmitoylated. Depalmitoylated by CPT1C and upon L-glutamate stimulation. ZDHHC3/GODZ specifically palmitoylates Cys-603, which leads to Golgi retention and decreased cell surface expression. In contrast, Cys-829 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis.
Function
Ionotropic glutamate receptor that functions as a ligand-gated cation channel, gated by L-glutamate and glutamatergic agonists such as alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA), quisqualic acid, and kainic acid. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse upon entry of monovalent and divalent cations such as sodium and calcium. The receptor then desensitizes rapidly and enters in a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG2 or CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of L-glutamate. Calcium (Ca(2+)) permeability depends on subunits composition and, heteromeric channels containing edited GRIA2 subunit are calcium-impermeable. Also permeable to other divalents cations such as strontium(2+) and magnesium(2+) and monovalent cations such as potassium(1+) and lithium(1+).
Cell MembraneMulti-Pass Membrane ProteinEndoplasmic Reticulum MembranePostsynaptic Cell MembranePostsynaptic Density MembraneCell ProjectionDendriteDendritic SpineEarly Endosome MembraneRecycling Endosome MembranePresynapseSynapseInteraction With Cacng2Cnih2 And Cnih3 Promotes Cell Surface ExpressionColocalizes With Pdlim4 In Early EndosomesDisplays A Somatodendritic Localization And Is Excluded From Axons In NeuronsLocalized To Cone Photoreceptor Pedicles
