| Applications: | ELISA |
| Reactivity: | Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: | 0.116ng/mL |
| Detection Limit: | 0.312-20ng/mL |
| Short Description: | This GHR Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse serum, plasma, tissue homogenates or other biological fluids.. |
| Storage Instruction: | Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: | 4.5 hrs |
| Gene Symbol: | Ghr |
| Gene ID: | 14600 |
| Uniprot ID: | GHR_MOUSE |
| Sample Type: | serum, plasma, tissue homogenates or other biological fluids. |
| Tissue Specificity | Expressed in all tissues tested including, liver, heart, adipose tissue, mammary gland, testes, ovary, brain, kidney and muscle. Highest levels in liver. |
| Post Translational Modifications | The soluble form (GHBP) is produced by phorbol ester-promoted proteolytic cleavage at the cell surface (shedding) by ADAM17/TACE. Shedding is inhibited by growth hormone (GH) binding to the receptor probably due to a conformational change in GHR rendering the receptor inaccessible to ADAM17. On GH binding, phosphorylated on tyrosine residues in the cytoplasmic domain by JAK2. Ubiquitinated by the ECS(SOCS2) complex following ligand-binding and phosphorylation by JAK2, leading to its degradation by the proteasome. Regulation by the ECS(SOCS2) complex acts as a negative feedback loop of growth hormone receptor signaling. Ubiquitination is not sufficient for GHR internalization. |
| Function | Receptor for pituitary gland growth hormone (GH1) involved in regulating postnatal body growth. On ligand binding, couples to the JAK2/STAT5 pathway. Growth hormone-binding protein: The soluble form (GHBP) acts as a reservoir of growth hormone in plasma and may be a modulator/inhibitor of GH signaling. |
| Protein Name | Growth Hormone Receptor Gh Receptor Somatotropin Receptor Cleaved Into - Growth Hormone-Binding Protein Gh-Binding Protein Ghbp Serum-Binding Protein |
| Database Links | Reactome: R-MMU-1170546 Reactome: -MMU-982772 |
| Cellular Localisation | Cell Membrane Single-Pass Type I Membrane Protein On Growth Hormone Binding Ghr Is Ubiquitinated Internalized Down-Regulated And Transported Into A Degradative Or Non-Degradative Pathway Growth Hormone-Binding Protein: Secreted Complexed To A Substantial Fraction Of Circulating Gh |
| Alternative ELISA Names | Growth Hormone Receptor ELISA kit Gh Receptor ELISA kit Somatotropin Receptor Cleaved Into - Growth Hormone-Binding Protein ELISA kit Gh-Binding Protein ELISA kit Ghbp ELISA kit Serum-Binding Protein ELISA kit Ghr ELISA kit |
| output |
Information sourced from Uniprot.org
