Mouse GCK (Glucokinase) Sandwich ELISA Kit Ready-To-Use Kit (STJE0015472)
SPECIFICATIONS
General Information
| Short Description | This GCK Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse serum, plasma, tissue homogenates or other biological fluids.. |
| Applications | ELISA |
| Reactivity | Mouse |
| Sensitivity | 0.054ng/mL |
| Detection Limit | 0.156-10ng/mL |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Storage Instruction | The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time | 3 hrs |
Target Information
| Gene Symbol | Gck |
| Gene ID | 103988 |
| Uniprot ID | HXK4_MOUSE |
| Immunogen Region | Ready-To-Use |
| Sample Type | serum, plasma, tissue homogenates or other biological fluids. |
Additional Info
| Tissue Specificity | |
| Post Translational Modifications | |
| Function | Catalyzes the phosphorylation of hexose, such as D-glucose, D-fructose and D-mannose, to hexose 6-phosphate (D-glucose 6-phosphate, D-fructose 6-phosphate and D-mannose 6-phosphate, respectively). Compared to other hexokinases, has a weak affinity for D-glucose, and is effective only when glucose is abundant. Mainly expressed in pancreatic beta cells and the liver and constitutes a rate-limiting step in glucose metabolism in these tissues. Since insulin secretion parallels glucose metabolism and the low glucose affinity of GCK ensures that it can change its enzymatic activity within the physiological range of glucose concentrations, GCK acts as a glucose sensor in the pancreatic beta cell. In pancreas, plays an important role in modulating insulin secretion. In liver, helps to facilitate the uptake and conversion of glucose by acting as an insulin-sensitive determinant of hepatic glucose usage. Required to provide D-glucose 6-phosphate for the synthesis of glycogen. Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate. |
| Protein Name | Hexokinase-4Hk4GlucokinaseHexokinase Type IvHk IvHexokinase-D |
| Database Links | Reactome: R-MMU-170822 P52792-2Reactome: -MMU-70171 P52792-2 |
| Cellular Localisation | CytoplasmNucleusMitochondrionUnder Low Glucose ConcentrationsGck Associates With Gckr And The Inactive Complex Is Recruited To The Hepatocyte Nucleus |
| Alternative ELISA Names | Hexokinase-4 ELISA kitHk4 ELISA kitGlucokinase ELISA kitHexokinase Type Iv ELISA kitHk Iv ELISA kitHexokinase-D ELISA kitGck ELISA kitGk ELISA kit |
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Information sourced from Uniprot.org