Mouse F9 (Coagulation Factor Ix) Antibody Pair (STJAP00287)
General Information
| Short Description | This F9 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Rabbit/Rabbit |
| Reactivity | Mouse |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Clonality | Polyclonal/Polyclonal |
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Protein A & Antigen Affinity.Detection Purification: Protein A & Antigen Affinity. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4.Detection Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
Target Information
| Gene Symbol | F9 |
| Gene ID | 14071 |
| Uniprot ID | FA9_MOUSE |
| Immunogen | Capture immunogen: Recombinant Mouse F9 protein.Detection Immunogen: Recombinant Mouse F9 protein. |
Additional Info
| Post Translational Modifications | Activated by factor XIa, which excises the activation peptide. The propeptide can also be removed by snake venom protease. Activated by coagulation factor VIIa-tissue factor (F7-F3) complex in calcium-dependent manner. The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains. Predominantly O-glucosylated at Ser-99 by POGLUT1 in vitro. |
| Function | Factor IX is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca(2+) ions, phospholipids, and factor VIIIa. |
| Protein Name | Coagulation Factor IxChristmas Factor Cleaved Into - Coagulation Factor Ixa Light Chain - Coagulation Factor Ixa Heavy Chain |
| Database Links | Reactome: R-MMU-140834Reactome: -MMU-140837Reactome: -MMU-159740Reactome: -MMU-159763Reactome: -MMU-159782 |
| Cellular Localisation | Secreted |
| Alternative Antibody Pair Names | Coagulation Factor Ix antibody pairChristmas Factor Cleaved Into - Coagulation Factor Ixa Light Chain - Coagulation Factor Ixa Heavy Chain antibody pairF9 antibody pairCf9 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Mouse F9 (Coagulation Factor Ix) Antibody Pair (STJAP00287)
This antibody pair detects mouse Coagulation Factor IX (F9), corresponding to UniProt entry P16294. Reactivity has been confirmed for mouse only.
Both antibodies are rabbit polyclonal antibodies supplied at 0.5 mg/mL. The capture antibody is unconjugated, while the detection antibody is biotinylated. Both are formulated in PBS with 0.04% Proclin 300 and 50% glycerol at pH 7.4.
This pair has been validated for use in ELISA. No other assay formats are listed in the product specifications.
Both the capture and detection antibodies were raised against recombinant mouse F9 protein. Both antibodies were purified using a combination of Protein A and antigen affinity purification methods.
Both components should be stored at -20°C for long-term storage. Repeated freeze-thaw cycles should be avoided to maintain product integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.