Mouse F9 (Coagulation Factor 9) CLIA Kit for Quantitative Detection

SPECIFICATIONS
STJC0001160
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General Information

Short DescriptionThis mouse F9 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids.
ApplicationsCLIA
ReactivityMouse
Sensitivity18.75pg/mL
Detection Limit31.25~2000pg/mL
NoteFOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Product Properties

Storage InstructionIf unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time3.5h
DetectionChemiluminescence

Target Information

Gene SymbolF9
Gene ID14071
Uniprot IDFA9_MOUSE
SpecificityThis kit recognizes Mouse F9 in samples. No significant cross-reactivity or interference between Mouse F9 and analogues was observed.
Sample TypeSerum, plasma and other biological fluids

Additional Info

Tissue Specificity Detected in liver.
Post Translational Modifications Activated by factor XIa, which excises the activation peptide. The propeptide can also be removed by snake venom protease. Activated by coagulation factor VIIa-tissue factor (F7-F3) complex in calcium-dependent manner. The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains. Predominantly O-glucosylated at Ser-99 by POGLUT1 in vitro.
Function Factor IX is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca(2+) ions, phospholipids, and factor VIIIa.
Protein Name Coagulation Factor Ix
Christmas Factor Cleaved Into - Coagulation Factor Ixa Light Chain - Coagulation Factor Ixa Heavy Chain
Database Links Reactome: R-MMU-140834
Reactome: -MMU-140837
Reactome: -MMU-159740
Reactome: -MMU-159763
Reactome: -MMU-159782
Cellular Localisation Secreted
Alternative CLIA Names Coagulation Factor Ix CLIA kit
Christmas Factor Cleaved Into - Coagulation Factor Ixa Light Chain - Coagulation Factor Ixa Heavy Chain CLIA kit
F9 CLIA kit
Cf9 CLIA kit
Specificity This kit recognizes Mouse F9 in samples. No significant cross-reactivity or interference between Mouse F9 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 90-103 95
EDTA plasma(n=8) 89-101 94
Cell culture media(n=8) 99-113 105

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 91.70 258.28 824.60 99.06 233.95 752.79
Standard deviation 9.90 21.28 72.81 12.61 25.13 81.53
CV (%) 10.80 8.24 8.83 12.73 10.74 10.83

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