Mouse F8 (Coagulation factor VIII) Sandwich ELISA Kit (STJE0004428)

Name: Mouse F8 (Coagulation factor VIII) Sandwich ELISA Kit (STJE0004428)
SKU: STJE0004428
Availability: InStock

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SPECIFICATIONS

STJE0004428

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Short Description	This F8 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse serum, plasma and other biological fluids.
Applications	ELISA
Reactivity	Mouse
Sensitivity	0.19ng/mL
Detection Limit	0.31~20ng/mL
Note	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Storage Instruction	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time	3.5h
Detection	Colormetric

Gene Symbol	F8
Gene ID	14069
Uniprot ID	FA8_MOUSE
Specificity	This kit recognizes Mouse F8 in samples. No significant cross-reactivity or interference between Mouse F8 and analogues was observed.
Sample Type	Serum, plasma and other biological fluids

Post Translational Modifications	The binding of vWF and activation depend on the sulfation of Tyr-1669. Proteolytically cleaved by cathepsin CTSG to produce a partially activated form.
Function	Factor VIII, along with calcium and phospholipid, acts as a cofactor for factor IXa when it converts factor X to the activated form, factor Xa.
Protein Name	Coagulation Factor Viii Procoagulant Component
Database Links	Reactome: R-MMU-114608 Reactome: -MMU-140837 Reactome: -MMU-140875 Reactome: -MMU-163841 Reactome: -MMU-204005 Reactome: -MMU-5694530
Cellular Localisation	Secreted Extracellular Space
Alternative ELISA Names	Coagulation Factor Viii ELISA kit Procoagulant Component ELISA kit F8 ELISA kit Cf8 ELISA kit F8c ELISA kit
Specificity	This kit recognizes Mouse F8 in samples. No significant cross-reactivity or interference between Mouse F8 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (ng/mL)	1.03	3.17	9.68	1.01	2.93	10.02
Standard deviation	0.05	0.15	0.48	0.06	0.17	0.53
CV (%)	4.85	4.73	4.96	5.94	5.80	5.29