• Mouse F12 (Coagulation Factor 7) CLIA Kit (STJC0001162)

Mouse F12 (Coagulation Factor 7) CLIA Kit (STJC0001162)

SKU:
STJC0001162

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Applications: CLIA
Reactivity: Mouse
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 18.75pg/mL
Detection Limit: 31.25~2000pg/mL
Short Description: This mouse F12 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: F12
Gene ID: 58992
Uniprot ID: FA12_MOUSE
Specificity: This kit recognizes Mouse F12 in samples. No significant cross-reactivity or interference between Mouse F12 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications O- and N-glycosylated.
Function Factor XII is a serum glycoprotein that participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then trypsin cleaves it to beta-factor XIIa. Alpha-factor XIIa activates factor XI to factor XIa.
Protein Name Coagulation Factor Xii
Hageman Factor
Haf Cleaved Into - Coagulation Factor Xiia Heavy Chain - Coagulation Factor Xiia Light Chain
Database Links Reactome: R-MMU-140837
Cellular Localisation Secreted
Alternative CLIA Names Coagulation Factor Xii CLIA kit
Hageman Factor CLIA kit
Haf Cleaved Into - Coagulation Factor Xiia Heavy Chain - Coagulation Factor Xiia Light Chain CLIA kit
F12 CLIA kit
Specificity This kit recognizes Mouse F12 in samples. No significant cross-reactivity or interference between Mouse F12 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 94-109 101
EDTA plasma(n=8) 86-98 91
Cell culture media(n=8) 84-95 90

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 100.16 183.72 730.37 104.63 179.23 800.53
Standard deviation 9.58 19.93 45.87 12.31 16.24 69.89
CV (%) 9.56 10.85 6.28 11.77 9.06 8.73
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance