Mouse F12 (Coagulation Factor 7) CLIA Kit for Quantitative Detection

Name: Mouse F12 (Coagulation Factor 7) CLIA Kit for Quantitative Detection
SKU: STJC0001162
Availability: InStock

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SPECIFICATIONS

STJC0001162

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Short Description	This mouse F12 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids.
Applications	CLIA
Reactivity	Mouse
Sensitivity	18.75pg/mL
Detection Limit	31.25~2000pg/mL
Note	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Storage Instruction	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time	3.5h
Detection	Chemiluminescence

Gene Symbol	F12
Gene ID	58992
Uniprot ID	FA12_MOUSE
Specificity	This kit recognizes Mouse F12 in samples. No significant cross-reactivity or interference between Mouse F12 and analogues was observed.
Sample Type	Serum, plasma and other biological fluids

Post Translational Modifications	O- and N-glycosylated.
Function	Factor XII is a serum glycoprotein that participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then trypsin cleaves it to beta-factor XIIa. Alpha-factor XIIa activates factor XI to factor XIa.
Protein Name	Coagulation Factor Xii Hageman Factor Haf Cleaved Into - Coagulation Factor Xiia Heavy Chain - Coagulation Factor Xiia Light Chain
Database Links	Reactome: R-MMU-140837
Cellular Localisation	Secreted
Alternative CLIA Names	Coagulation Factor Xii CLIA kit Hageman Factor CLIA kit Haf Cleaved Into - Coagulation Factor Xiia Heavy Chain - Coagulation Factor Xiia Light Chain CLIA kit F12 CLIA kit
Specificity	This kit recognizes Mouse F12 in samples. No significant cross-reactivity or interference between Mouse F12 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro CLIA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent A	1 vial, 5 mL	2-8℃ (Protect from light)
Substrate Reagent B	1 vial, 5 mL	2-8℃ (Protect from light)
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	100.16	183.72	730.37	104.63	179.23	800.53
Standard deviation	9.58	19.93	45.87	12.31	16.24	69.89
CV (%)	9.56	10.85	6.28	11.77	9.06	8.73