Mouse Eng (Endoglin) Sandwich ELISA Kit (STJE0004980)

Name: Mouse Eng (Endoglin) Sandwich ELISA Kit (STJE0004980)
SKU: STJE0004980
Availability: InStock

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SPECIFICATIONS

STJE0004980

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Short Description	This Eng Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse serum, plasma and other biological fluids.
Applications	ELISA
Reactivity	Mouse
Sensitivity	37.5pg/mL
Detection Limit	62.5~4000pg/mL
Note	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Storage Instruction	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time	3.5h
Detection	Colormetric

Gene Symbol	Eng
Gene ID	13805
Uniprot ID	EGLN_MOUSE
Specificity	This kit recognizes Mouse ENG in samples. No significant cross-reactivity or interference between Mouse ENG and analogues was observed.
Sample Type	Serum, plasma and other biological fluids

Function	Vascular endothelium glycoprotein that plays an important role in the regulation of angiogenesis. Required for normal structure and integrity of adult vasculature. Regulates the migration of vascular endothelial cells. Required for normal extraembryonic angiogenesis and for embryonic heart development. May regulate endothelial cell shape changes in response to blood flow, which drive vascular remodeling and establishment of normal vascular morphology during angiogenesis. May play a role in the binding of endothelial cells to integrins. Acts as a TGF-beta coreceptor and is involved in the TGF-beta/BMP signaling cascade that ultimately leads to the activation of SMAD transcription factors. Required for GDF2/BMP9 signaling through SMAD1 in endothelial cells and modulates TGFB1 signaling through SMAD3.
Protein Name	Endoglin Cell Surface Mj7/18 Antigen Cd Antigen Cd105
Database Links
Cellular Localisation	Cell Membrane Single-Pass Type I Membrane Protein
Alternative ELISA Names	Endoglin ELISA kit Cell Surface Mj7/18 Antigen ELISA kit Cd Antigen Cd105 ELISA kit Eng ELISA kit Edg ELISA kit
Specificity	This kit recognizes Mouse ENG in samples. No significant cross-reactivity or interference between Mouse ENG and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	200.09	481.89	1354.99	218.52	515.11	1433.13
Standard deviation	10.62	22.07	70.19	13.18	24.83	54.89
CV (%)	5.31	4.58	5.18	6.03	4.82	3.83