Mouse Endostatin protein (Recombinant) (C-His) (STJP013913)
SPECIFICATIONS
HostMammalian Cells
ConjugationUnconjugated
ImmunogenMus musculus (Mouse)
General Information
| Short Description | Recombinant-Mouse Endostatin-C-His protein was developed from mammalian cells and has a target region of C-His. For use in research applications. |
| Applications | ELISA/WB |
| Host | Mammalian Cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Dilution Range | Reconstitute in sterile water for a stock solution. |
| Formulation | Lyophilized from a 0.22 Mu m filtered solution in PBS, pH 7.4. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at-20 to-80°C for twelve months from the date of receipt. |
| Endotoxin | < 1 EU/µg as determined by LAL test. |
Target Information
| Gene Symbol | Col18a1 |
| Gene ID | 12822 |
| Uniprot ID | COIA1_MOUSE |
| Immunogen | Mus musculus (Mouse) |
| Immunogen Region | His1591-Lys1774 |
Additional Info
| Post Translational Modifications | Prolines at the third position of the tripeptide repeating unit (G-X-Y) of the triple-helical regions are hydroxylated. Undergoes proteolytic processing by CTSL/cathepsin-L and elastase-like proteases to generate both non-collagenous domain 1 trimers and endostatin monomers. In tissue extracts (brain, skeletal muscle, heart, kidney, testis and liver) predominantly bands of approximately 38 kDa are detected.recombinant non-collagenous domain 1 shows similar mobility. In vitro, several proteolytic cleavage sites in the non-collagenous domain 1 hinge region generating different endostatin-like peptides are reported. |
| Function | Probably plays a major role in determining the retinal structure as well as in the closure of the neural tube. Non-collagenous domain 1: May regulate extracellular matrix-dependent motility and morphogenesis of endothelial and non-endothelial cells.the function requires homotrimerization and implicates MAPK signaling. Endostatin: Potently inhibits endothelial cell proliferation and angiogenesis. May inhibit angiogenesis by binding to the heparan sulfate proteoglycans involved in growth factor signaling. Inhibits VEGFA isoform VEGF165-induced endothelial cell proliferation and migration. Seems to inhibit VEGFA-mediated signaling by blocking the interaction of VEGFA to its receptor KDR/VEGFR2. Modulates endothelial cell migration in an integrin-dependent manner implicating integrin ITGA5:ITGB1 and to a lesser extent ITGAV:ITGB3 and ITGAV:ITGB5. May negatively regulate the activity of homotrimeric non-collagenous domain 1. |
| Protein Name | Collagen Alpha-1(Xviii Chain Cleaved Into - Endostatin - Non-Collagenous Domain 1Nc1 |
| Database Links | Reactome: R-MMU-1442490Reactome: -MMU-1592389Reactome: -MMU-1650814Reactome: -MMU-2022090Reactome: -MMU-216083Reactome: -MMU-8948216 |
| Cellular Localisation | SecretedExtracellular SpaceExtracellular MatrixBasement MembraneNon-Collagenous Domain 1: SecretedEndostatin: Secreted |
| Alternative Protein Names | Collagen Alpha-1(Xviii Chain Cleaved Into - Endostatin - Non-Collagenous Domain 1 proteinNc1 proteinCol18a1 protein |
Information sourced from Uniprot.org