Mouse CTLA4 (Cytotoxic T-Lymphocyte Protein 4) Antibody Pair (STJAP00306)
General Information
| Short Description | This CTLA4 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Goat/Goat |
| Reactivity | Mouse |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Clonality | Polyclonal/Polyclonal |
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Antigen Affinity.Detection Purification: Antigen Affinity. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4.Detection Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
Target Information
| Gene Symbol | Ctla4 |
| Gene ID | 12477 |
| Uniprot ID | CTLA4_MOUSE |
| Immunogen | Capture immunogen: Recombinant Mouse CTLA4 protein.Detection Immunogen: Recombinant Mouse CTLA4 protein. |
Additional Info
| Post Translational Modifications | N-glycosylation is important for dimerization. Phosphorylation at Tyr-201 prevents binding to the AP-2 adapter complex, blocks endocytosis, and leads to retention of CTLA4 on the cell surface. |
| Function | Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28. |
| Protein Name | Cytotoxic T-Lymphocyte Protein 4Cytotoxic T-Lymphocyte-Associated Antigen 4Ctla-4Cd Antigen Cd152 |
| Database Links | Reactome: R-MMU-389356Reactome: -MMU-389513 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinExists Primarily An Intracellular Antigen Whose Surface Expression Is Tightly Regulated By Restricted Trafficking To The Cell Surface And Rapid Internalization |
| Alternative Antibody Pair Names | Cytotoxic T-Lymphocyte Protein 4 antibody pairCytotoxic T-Lymphocyte-Associated Antigen 4 antibody pairCtla-4 antibody pairCd Antigen Cd152 antibody pairCtla4 antibody pairCd152 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Mouse CTLA4 (Cytotoxic T-Lymphocyte Protein 4) Antibody Pair (STJAP00306)
This antibody pair detects mouse CTLA4 (Cytotoxic T-Lymphocyte Protein 4), corresponding to UniProt entry P09793. Reactivity has been confirmed for mouse samples only.
Both antibodies are polyclonal, raised in goat, and supplied at 0.5 mg/mL. The capture antibody is unconjugated, while the detection antibody is biotinylated. Both are formulated in PBS with 0.04% Proclin 300 and 50% glycerol at pH 7.4.
This antibody pair is validated for use in ELISA. No other assay formats are listed in the product specifications.
Both antibodies were raised against recombinant mouse CTLA4 protein. Both capture and detection antibodies were subsequently purified by antigen affinity chromatography.
Both components should be stored at -20 degrees Celsius for long-term storage. Repeated freeze-thaw cycles should be avoided to maintain reagent integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.