Mouse CFH (Complement Factor H) Antibody Pair (STJAP00278)
General Information
| Short Description | This CFH antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Sheep/Sheep |
| Reactivity | Mouse |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Clonality | Polyclonal/Polyclonal |
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Antigen Affinity.Detection Purification: Antigen Affinity. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4.Detection Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
Target Information
| Gene Symbol | Cfh |
| Gene ID | 12628 |
| Uniprot ID | CFAH_MOUSE |
| Immunogen | Capture immunogen: Recombinant Mouse CFH protein.Detection Immunogen: Recombinant Mouse CFH protein. |
Additional Info
| Post Translational Modifications | Sulfated on tyrosine residues. |
| Function | Glycoprotein that plays an essential role in maintaining a well-balanced immune response by modulating complement activation. Acts as a soluble inhibitor of complement, where its binding to self markers such as glycan structures prevents complement activation and amplification on cell surfaces. Accelerates the decay of the complement alternative pathway (AP) C3 convertase C3bBb, thus preventing local formation of more C3b, the central player of the complement amplification loop. As a cofactor of the serine protease factor I, CFH also regulates proteolytic degradation of already-deposited C3b. In addition, mediates several cellular responses through interaction with specific receptors. For example, interacts with CR3/ITGAM receptor and thereby mediates the adhesion of human neutrophils to different pathogens. In turn, these pathogens are phagocytosed and destroyed. |
| Protein Name | Complement Factor HProtein Beta-1-H |
| Database Links | Reactome: R-MMU-977606 |
| Cellular Localisation | Secreted |
| Alternative Antibody Pair Names | Complement Factor H antibody pairProtein Beta-1-H antibody pairCfh antibody pairHf1 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Mouse CFH (Complement Factor H) Antibody Pair (STJAP00278)
This antibody pair is designed to detect mouse Complement Factor H (CFH), corresponding to UniProt accession P06909. Reactivity has been confirmed for mouse samples only.
Both antibodies are sheep-raised polyclonals purified by antigen affinity. The capture antibody is unconjugated, while the detection antibody is biotinylated. Both are supplied at 0.5 mg/mL in PBS with 0.04% Proclin 300 and 50% glycerol, pH 7.4.
This pair has been validated for use in ELISA. No other assay formats are specified for this product.
Both the capture and detection antibodies were raised against recombinant mouse CFH protein. No specific immunogen region is defined in the product details.
Both components should be stored at -20 degrees Celsius for long-term storage. Freeze-thaw cycles should be avoided to maintain antibody integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.