Mouse CD95 (CD95) Antibody Pair (STJAP00947)
General Information
| Short Description | This CD95 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Goat/Goat |
| Reactivity | Mouse |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL/0.5-4ug/mL |
| Dilution Range | 1:1000-1:10000 |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
| Detection | Colorimetric method;ELISA;Sandwich |
Target Information
| Gene Symbol | Fas |
| Gene ID | 14102 |
| Uniprot ID | TNR6_MOUSE |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Post Translational Modifications | Palmitoylated. Palmitoylation by ZDHHC7 prevents the lysosomal degradation of FAS regulating its expression at the plasma membrane. |
| Function | Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase CASP8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs CASP8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. |
| Protein Name | Tumor Necrosis Factor Receptor Superfamily Member 6Apo-1 AntigenApoptosis-Mediating Surface Antigen FasFaslg ReceptorCd Antigen Cd95 |
| Database Links | Reactome: R-MMU-3371378Reactome: -MMU-5218900Reactome: -MMU-69416Reactome: -MMU-75157 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinMembrane Raft |
| Alternative Antibody Pair Names | Tumor Necrosis Factor Receptor Superfamily Member 6 antibody pairApo-1 Antigen antibody pairApoptosis-Mediating Surface Antigen Fas antibody pairFaslg Receptor antibody pairCd Antigen Cd95 antibody pairFas antibody pairApt1 antibody pairTnfrsf6 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Mouse CD95 (CD95) Antibody Pair (STJAP00947)
This antibody pair detects mouse CD95 (also known as Fas, UniProt P25446) and is validated for reactivity in mouse samples including serum, plasma, and other biological fluids.
The capture antibody is supplied unconjugated at 0.5 mg/mL, and the detection antibody is supplied biotinylated at a concentration of 0.5 to 4 ug/mL. Both antibodies are raised in goat.
This pair is validated for use in sandwich ELISA with colorimetric detection. No other assay formats are specified for this product.
The recommended working dilution range is 1:1000 to 1:10000. Users should perform empirical optimization within this range for their specific experimental conditions.
This pair is suitable for use with serum, plasma, and other biological fluids from mouse origin.
Both components should be stored at -20 degrees Celsius for long-term storage. Repeated freeze-thaw cycles should be avoided to maintain antibody integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.