Mouse CD163 protein (Recombinant) (C-His) (STJP002051)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Mouse CD163 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met1-Thr1045) of mouse CD163 (Accession #NP_444324.2) fused with 8×His tag at the C-terminus.
General Information
| Short Description | Recombinant-Mouse CD163-C-His protein was developed in hek293 cells using the region Met1-Thr1045. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method | <0.1 EU/Mu g |
Target Information
| Gene Symbol | Cd163 |
| Gene ID | 93671 |
| Uniprot ID | C163A_MOUSE |
| Immunogen | Recombinant Mouse CD163 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met1-Thr1045) of mouse CD163 (Accession #NP_444324.2) fused with 8×His tag at the C-terminus. |
| Immunogen Region | Met1-Thr1045 |
| Immunogen Sequence | MGGHRMVLLGGAGSPGCKRF VHLGFFVVAVSSLLSASAVT NAPGEMKKELRLAGGENNCS GRVELKIHDKWGTVCSNGWS MNEVSVVCQQLGCPTSIKAL GWANSSAGSGYIWMDKVSCT GNESALWDCKHDGWGKHNCT HEKDAGVTCSDGSNLEMRLV NSAGHRCLGRVEIKFQGKWG TVCDDNFSKDHASVICKQLG CGSAISFSGSAKLGAGSGPI WLDDLACNGNESALWDCKH |
Additional Info
| Post Translational Modifications | A soluble form (sCD163) is produced by proteolytic shedding which can be induced by lipopolysaccharide, phorbol ester and Fc region of immunoglobulin gamma. This cleavage is dependent on protein kinase C and tyrosine kinases and can be blocked by protease inhibitors. The shedding is inhibited by the tissue inhibitor of metalloproteinase TIMP3, and thus probably induced by membrane-bound metalloproteinases ADAMs. |
| Function | Involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. After shedding, the soluble form (sCD163) may play an anti-inflammatory role. |
| Protein Name | Scavenger Receptor Cysteine-Rich Type 1 Protein M130Cd Antigen Cd163 Cleaved Into - Soluble Cd163Scd163 |
| Database Links | Reactome: R-MMU-2168880 |
| Cellular Localisation | Soluble Cd163: SecretedCell MembraneSingle-Pass Type I Membrane Protein |
| Alternative Protein Names | Scavenger Receptor Cysteine-Rich Type 1 Protein M130 proteinCd Antigen Cd163 Cleaved Into - Soluble Cd163 proteinScd163 proteinCd163 proteinM130 protein |
Information sourced from Uniprot.org