Mouse CD147 (CD147) Antibody Pair (STJAP00765)
General Information
| Short Description | This CD147 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the. |
| Applications | ELISA |
| Host | Rabbit/Rabbit |
| Reactivity | Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Monoclonal |
| Clone ID | R19-6D-6/R34-5G-5 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | Determined by end user |
| Formulation | 0.01M PBS, pH 7.2 |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | Bsg |
| Gene ID | 12215 |
| Uniprot ID | BASI_MOUSE |
| Immunogen | Recombinant protein |
Additional Info
| Tissue Specificity | Isoform 1: Retina-specific. Expressed in both rods and cones (at protein level). Isoform 2: Testis and caput, corpus and cauda epididymides (at protein level). Expressed in the brain, lung, liver, kidney, heart, spleen, uterus, retina and skeletal muscle. |
| Post Translational Modifications | Isoform 1: N-glycosylated. Isoform 2: N-glycosylated. During spermatogenesis, probably deglycosylated during epididymal transit. |
| Function | Isoform 1: Essential for normal retinal maturation and development. Acts as a retinal cell surface receptor for NXNL1 and plays an important role in NXNL1-mediated survival of retinal cone photoreceptors. In association with glucose transporter SLC16A1/GLUT1 and NXNL1, promotes retinal cone survival by enhancing aerobic glycolysis and accelerating the entry of glucose into photoreceptors. Isoform 2: Signaling receptor for cyclophilins, essential for PPIA/CYPA and PPIB/CYPB-dependent signaling related to chemotaxis and adhesion of immune cells. Plays an important role in targeting the monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to the plasma membrane. Acts as a coreceptor for vascular endothelial growth factor receptor 2 (KDR/VEGFR2) in endothelial cells enhancing its VEGFA-mediated activation and downstream signaling. Promotes angiogenesis through EPAS1/HIF2A-mediated up-regulation of VEGFA and KDR/VEGFR2 in endothelial cells. Plays an important role in spermatogenesis.mediates interactions between germ cells and Sertoli cell and is essential for the development/differentiation of germ cells to round spermatids. |
| Protein Name | BasiginBasic Immunoglobulin SuperfamilyHt7 AntigenMembrane Glycoprotein Gp42Cd Antigen Cd147 |
| Database Links | Reactome: R-MMU-1474228Reactome: -MMU-210991Reactome: -MMU-216083Reactome: -MMU-433692Reactome: -MMU-9749641 |
| Cellular Localisation | Isoform 1: Cell MembraneSingle-Pass Type I Membrane ProteinPhotoreceptor Inner SegmentCell ProjectionCiliumPhotoreceptor Outer SegmentIsoform 2: Cell MembraneEndoplasmic Reticulum MembraneBasolateral Cell Membrane |
| Alternative Antibody Pair Names | Basigin antibody pairBasic Immunoglobulin Superfamily antibody pairHt7 Antigen antibody pairMembrane Glycoprotein Gp42 antibody pairCd Antigen Cd147 antibody pairBsg antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Mouse CD147 (CD147) Antibody Pair (STJAP00765)
This antibody pair is designed to detect mouse CD147 (UniProt P18572) and has confirmed reactivity in mouse. It is not validated for use with human or other species samples.
This antibody pair is validated for use in ELISA. It has not been validated for other assay formats such as lateral flow or multiplex immunoassay.
Both antibodies are rabbit monoclonal IgGs (clone IDs R19-6D-6 and R34-5G-5), supplied unconjugated at 1 mg/mL in 0.01M PBS, pH 7.2, and purified by affinity chromatography. Neither antibody carries a conjugate or tag, so downstream labeling or use of a conjugated secondary reagent will be required for detection.
Optimal working concentrations are to be determined by the end user and are not specified in the product documentation. Titration of both antibodies is recommended when setting up the ELISA for the first time.
Both antibodies should be stored at 4°C for short-term use or aliquoted and stored at -20°C for long-term storage. Repeated freeze/thaw cycles should be avoided to preserve antibody integrity.
Both antibodies were raised against a recombinant protein corresponding to mouse CD147, though the specific immunogen region is not defined in the current product documentation. Researchers requiring immunogen region details should contact technical support directly.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.