Mouse CD117 (CD117) Antibody Pair (STJAP00954)
SPECIFICATIONS
HostRabbit/Rabbit
ConjugationUnconjugated/Biotin
General Information
| Short Description | This CD117 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Rabbit/Rabbit |
| Reactivity | Mouse |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL/0.5-4ug/mL |
| Dilution Range | 1:1000-1:10000 |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
| Detection | Colorimetric method;ELISA;Sandwich |
Target Information
| Gene Symbol | Kit |
| Gene ID | 16590 |
| Uniprot ID | KIT_MOUSE |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Post Translational Modifications | Ubiquitinated by SOCS6. KIT is rapidly ubiquitinated after autophosphorylation induced by KITLG/SCF binding, leading to internalization and degradation. Autophosphorylated on tyrosine residues. KITLG/SCF binding promotes autophosphorylation of isoform 1 and isoform 2. Isoform 1 shows low levels of tyrosine phosphorylation in the absence of added KITLG/SCF, while isoform 2 requires stimulation by KITLG/SCF for phosphorylation (in vitro). Phosphorylation of Tyr-573 is required for interaction with PTPN6/SHP-1. Phosphorylation of Tyr-571 is required for interaction with PTPN11/SHP-2. Phosphorylated tyrosine residues are important for interaction with specific binding partners. |
| Function | Tyrosine-protein kinase that acts as a cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1. |
| Protein Name | Mast/Stem Cell Growth Factor Receptor KitScfrProto-Oncogene C-KitTyrosine-Protein Kinase KitCd Antigen Cd117 |
| Database Links | Reactome: R-MMU-1257604Reactome: -MMU-1433557Reactome: -MMU-1433559Reactome: -MMU-5673001Reactome: -MMU-6811558Reactome: -MMU-9856649 |
| Cellular Localisation | Isoform 1: Cell MembraneSingle-Pass Type I Membrane ProteinIsoform 2: Cell MembraneIsoform 3: CytoplasmDetected In The Cytoplasm Of SpermatozoaEspecially In The Equatorial And Subacrosomal Region Of The Sperm Head |
| Alternative Antibody Pair Names | Mast/Stem Cell Growth Factor Receptor Kit antibody pairScfr antibody pairProto-Oncogene C-Kit antibody pairTyrosine-Protein Kinase Kit antibody pairCd Antigen Cd117 antibody pairKit antibody pairSl antibody pair |
Information sourced from Uniprot.org