Mouse CD10 protein (Recombinant) (STJP001882)
SPECIFICATIONS
HostHEK293
ConjugationUnconjugated
ImmunogenOptimized DNA sequence encoding extracellular domain of mouse Neprilysin fused to a N-term polyHis tag was expressed in HEK293 cells. NA
General Information
| Short Description | Recombinant-Mouse CD10-protein was developed from hek293. For use in research applications. |
| Host | HEK293 |
| Note | STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | 0.2 Mu m filtered solution in 50mM Tris, 100mM NaCL, pH8.0. |
| Storage Instruction | Can be stored in working aliquots at 2°C-8°C C for one month, or at-20°C C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. NA |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). NA |
| Immunoreactivity | Measured by its ability to cleave flourogenic peptide substrate Mca-RPPGFSAFK (Dnp)-OH. The specific activity was measured to be >0.3nMole/min/ug NA |
Target Information
| Gene Symbol | MME |
| Gene ID | 4311 |
| Uniprot ID | NEP_HUMAN |
| Immunogen | Optimized DNA sequence encoding extracellular domain of mouse Neprilysin fused to a N-term polyHis tag was expressed in HEK293 cells. NA |
| Immunogen Region | ECD |
Additional Info
| Post Translational Modifications | Myristoylation is a determinant of membrane targeting. Glycosylation at Asn-628 is necessary both for surface expression and neutral endopeptidase activity. |
| Function | Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Catalyzes cleavage of bradykinin, substance P and neurotensin peptides. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF) and brain natriuretic factor (BNP(1-32)). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers. |
| Protein Name | NeprilysinAtriopeptidaseCommon Acute Lymphocytic Leukemia AntigenCallaEnkephalinaseNeutral Endopeptidase 24.11NepNeutral EndopeptidaseSkin Fibroblast ElastaseSfeCd Antigen Cd10 |
| Database Links | Reactome: R-HSA-2022377Reactome: R-HSA-5578768Reactome: R-HSA-6798695 |
| Cellular Localisation | Cell MembraneSingle-Pass Type Ii Membrane Protein |
| Alternative Protein Names | Neprilysin proteinAtriopeptidase proteinCommon Acute Lymphocytic Leukemia Antigen proteinCalla proteinEnkephalinase proteinNeutral Endopeptidase 24.11 proteinNep proteinNeutral Endopeptidase proteinSkin Fibroblast Elastase proteinSfe proteinCd Antigen Cd10 proteinMME proteinEPN protein |
Information sourced from Uniprot.org