Mouse CCL6 (Chemokine C-C-Motif Ligand 6) Sandwich ELISA Kit (STJE0019908)

Name: Mouse CCL6 (Chemokine C-C-Motif Ligand 6) Sandwich ELISA Kit (STJE0019908)
SKU: STJE0019908
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SPECIFICATIONS

STJE0019908

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Short Description	This CCL6 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse serum, plasma and other biological fluids.
Applications	ELISA
Reactivity	Mouse
Sensitivity	3.14 pg/mL
Detection Limit	7.81-500 pg/mL
Note	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Storage Instruction	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time	3.5h
Detection	Colormetric

Gene Symbol	Ccl6
Gene ID	20305
Uniprot ID	CCL6_MOUSE
Specificity	This kit recognizes Mouse CCL6 in samples. No significant cross-reactivity or interference between Mouse CCL6 and analogues was observed.
Sample Type	serum, plasma and other biological fluids

Tissue Specificity	Expressed in myelopoietic bone marrow cultures stimulated by GM-CSF.
Post Translational Modifications	The N-terminal is proteolytically cleaved by proteases associated with inflammatory responses. The processed forms CL6(22-95) and CCL6(23-95) show increase in CCR1-mediated signaling and chemotaxis assays in vitro.
Function	Chemotactic factor that attracts mostly macrophage, but it can also attract B cells, CD4(+) lymphocytes and eosinophils.
Protein Name	C-C Motif Chemokine 6 Protein C10 Small-Inducible Cytokine A6 Cleaved Into - Ccl6(22-95 - Ccl6(23-95
Database Links	Reactome: R-MMU-416476 Reactome: -MMU-418594 Reactome: -MMU-444473
Cellular Localisation	Secreted
Alternative ELISA Names	C-C Motif Chemokine 6 ELISA kit Protein C10 ELISA kit Small-Inducible Cytokine A6 Cleaved Into - Ccl6(22-95 - Ccl6(23-95 ELISA kit Ccl6 ELISA kit C10 ELISA kit Scya6 ELISA kit
Specificity	This kit recognizes Mouse CCL6 in samples. No significant cross-reactivity or interference between Mouse CCL6 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	24.58	56.71	235.32	24.88	53.18	229.29
Standard deviation	1.51	2.43	12.75	1.57	3.05	6.99
CV (%)	6.13	4.29	5.42	6.33	5.74	3.05