Mouse Ccl2 (C-C motif chemokine 2) Sandwich ELISA Kit (STJE0005317)

Name: Mouse Ccl2 (C-C motif chemokine 2) Sandwich ELISA Kit (STJE0005317)
SKU: STJE0005317
Availability: InStock

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SPECIFICATIONS

STJE0005317

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Short Description	This Ccl2 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse serum, plasma and other biological fluids.
Applications	ELISA
Reactivity	Mouse
Sensitivity	18.75pg/mL
Detection Limit	31.25~2000pg/mL
Note	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Storage Instruction	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time	3.5h
Detection	Colormetric

Gene Symbol	Ccl2
Gene ID	20296
Uniprot ID	CCL2_MOUSE
Specificity	This kit recognizes Mouse MCP-1 in samples. No significant cross-reactivity or interference between Mouse MCP-1 and analogues was observed.
Sample Type	Serum, plasma and other biological fluids

Function	Acts as a ligand for C-C chemokine receptor CCR2. Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions. Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils. Plays an important role in mediating peripheral nerve injury-induced neuropathic pain. Increases NMDA-mediated synaptic transmission in both dopamine D1 and D2 receptor-containing neurons, which may be caused by MAPK/ERK-dependent phosphorylation of GRIN2B/NMDAR2B.
Protein Name	C-C Motif Chemokine 2 Monocyte Chemoattractant Protein 1 Monocyte Chemotactic Protein 1 Mcp-1 Platelet-Derived Growth Factor-Inducible Protein Je Small-Inducible Cytokine A2
Database Links
Cellular Localisation	Secreted
Alternative ELISA Names	C-C Motif Chemokine 2 ELISA kit Monocyte Chemoattractant Protein 1 ELISA kit Monocyte Chemotactic Protein 1 ELISA kit Mcp-1 ELISA kit Platelet-Derived Growth Factor-Inducible Protein Je ELISA kit Small-Inducible Cytokine A2 ELISA kit Ccl2 ELISA kit Je ELISA kit Mcp1 ELISA kit Scya2 ELISA kit
Specificity	This kit recognizes Mouse MCP-1 in samples. No significant cross-reactivity or interference between Mouse MCP-1 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	94.05	210.98	994.91	88.91	244.77	840.43
Standard deviation	5.14	10.06	47.86	4.82	12.09	45.97
CV (%)	5.47	4.77	4.81	5.42	4.94	5.47