Mouse CCL12/MCP-5 (Monocyte Chemotactic Protein 5) Sandwich ELISA Kit (STJE0019725)

Name: Mouse CCL12/MCP-5 (Monocyte Chemotactic Protein 5) Sandwich ELISA Kit (STJE0019725)
SKU: STJE0019725
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SPECIFICATIONS

STJE0019725

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Short Description	This CCL12/MCP-5 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse serum, plasma and other biological fluids.
Applications	ELISA
Reactivity	Mouse
Sensitivity	3.97pg/mL
Detection Limit	7.81-500pg/mL
Note	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Storage Instruction	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time	3.5h
Detection	Colormetric

Gene Symbol	Ccl12
Gene ID	20293
Uniprot ID	CCL12_MOUSE
Specificity	This kit recognizes Mouse CCL12/MCP-5 in samples. No significant cross-reactivity or interference between Mouse CCL12/MCP-5 and analogues was observed.
Sample Type	Serum, plasma and other biological fluids

Function	Chemotactic factor that attracts eosinophils, monocytes, and lymphocytes but not neutrophils. Potent monocyte active chemokine that signals through CCR2. Involved in allergic inflammation and the host response to pathogens and may play a pivotal role during early stages of allergic lung inflammation.
Protein Name	C-C Motif Chemokine 12 Mcp-1-Related Chemokine Monocyte Chemoattractant Protein 5 Monocyte Chemotactic Protein 5 Mcp-5 Small-Inducible Cytokine A12
Database Links	Reactome: R-MMU-380108
Cellular Localisation	Secreted
Alternative ELISA Names	C-C Motif Chemokine 12 ELISA kit Mcp-1-Related Chemokine ELISA kit Monocyte Chemoattractant Protein 5 ELISA kit Monocyte Chemotactic Protein 5 ELISA kit Mcp-5 ELISA kit Small-Inducible Cytokine A12 ELISA kit Ccl12 ELISA kit Mcp5 ELISA kit Scya12 ELISA kit
Specificity	This kit recognizes Mouse CCL12/MCP-5 in samples. No significant cross-reactivity or interference between Mouse CCL12/MCP-5 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (50×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (50×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	25.00	56.06	239.73	26.91	54.37	233.69
Standard deviation	1.51	2.53	7.34	1.43	2.19	7.64
CV (%)	6.05	4.51	3.06	5.32	4.02	3.27