Mouse C5a (Complement C5) Antibody Pair (STJAP00270)
General Information
| Short Description | This C5a antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Rat/Goat |
| Reactivity | Mouse |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Polyclonal |
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Protein A or G.Detection Purification: Antigen Affinity. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4.Detection Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
Target Information
| Gene Symbol | C5 |
| Gene ID | 15139 |
| Uniprot ID | CO5_MOUSE |
| Immunogen | Capture immunogen: Recombinant Mouse C5a protein.Detection Immunogen: Recombinant Mouse C5a protein. |
Additional Info
| Function | Precursor of the C5a anaphylatoxin and complement C5b components of the complement pathways, which consist in a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system. Activated downstream of classical, alternative, lectin and GZMK complement pathways. Complement C5b: Component of the membrane attack complex (MAC), a multiprotein complex activated by the complement cascade, which inserts into a target cell membrane and forms a pore, leading to target cell membrane rupture and cell lysis. Complement C5b is generated following cleavage by C5 convertase and initiates formation of the MAC complex: C5b binds sequentially C6, C7, C8 and multiple copies of the pore-forming subunit C9. During MAC complex assembly, the C5b6 subcomplex, composed of complement C5b and C6, associates with the outer leaflet of target cell membrane, reducing the energy for membrane bending. C5a anaphylatoxin: Mediator of local inflammatory process released following cleavage by C5 convertase. Acts by binding to its receptor (C5AR1 or C5AR2), activating G protein-coupled receptor signaling and inducing a variety of responses including intracellular calcium release, contraction of smooth muscle, increased vascular permeability, and histamine release from mast cells and basophilic leukocytes. C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation. |
| Protein Name | Complement C5Hemolytic Complement Cleaved Into - Complement C5 Beta Chain - Complement C5 Alpha Chain - C5a Anaphylatoxin - Complement C5bComplement C5 Alpha' Chain |
| Database Links | Reactome: R-MMU-166665Reactome: -MMU-174577Reactome: -MMU-375276Reactome: -MMU-418594Reactome: -MMU-977606 |
| Cellular Localisation | SecretedComplement C5b: SecretedTarget Cell MembraneSecreted As Soluble ProteinInserts Into The Cell Membrane Of Target CellsC5a Anaphylatoxin: Secreted |
| Alternative Antibody Pair Names | Complement C5 antibody pairHemolytic Complement Cleaved Into - Complement C5 Beta Chain - Complement C5 Alpha Chain - C5a Anaphylatoxin - Complement C5b antibody pairComplement C5 Alpha' Chain antibody pairC5 antibody pairHc antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Mouse C5a (Complement C5) Antibody Pair (STJAP00270)
This antibody pair detects mouse C5a, a cleavage fragment of Complement C5 (UniProt P06684), and has confirmed reactivity in mouse. It is intended for in vitro research use only.
The capture antibody is a rat-derived monoclonal antibody supplied unconjugated at 0.5 mg/mL, while the detection antibody is a goat-derived polyclonal antibody supplied at 0.5 mg/mL conjugated to biotin. Both are formulated in PBS with 0.04% Proclin 300 and 50% glycerol at pH 7.4.
This pair has been validated for use in ELISA. No other assay formats are specified in the product documentation.
Both the capture and detection antibodies were raised against recombinant mouse C5a protein. The capture antibody was purified using Protein A or G, and the detection antibody was purified by antigen affinity chromatography.
Both components should be stored at -20 degrees C for long-term storage. Repeated freeze-thaw cycles should be avoided to maintain reagent integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.